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浙江大学学报（理学版）
浙江大学学报（理学版）
化学     
改进的三羟基吲哚法测定肾上腺素
1.浙江大学环境科学系,浙江 杭州 310028; 2.浙江大学附一医院,浙江 杭州 310003; 3.浙江省中医院,浙江 杭州 310006
Determination of epinephrine by an improved modification of the trihydroxyindole method.
1. Department of Environmental Science, Zhejiang University, Hangzhou 310028, China; 2. The First Hospital of Zhejiang University, Hangzhou 310003, China; 3. Traditional Medical Hospital of Zhejiang, Hangzhou 310006, China
 全文: PDF(143 KB)  
摘要： 本文利用改进的三羟基吲哚法测定肾上腺素. 实验表明,在pH=8.7的硼砂缓冲溶液中,肾上腺素用K3Fe(CN)6氧化后,加入巯基乙醇溶液,能稳定吲哚衍生物,从而可以在碱性条件下测定肾上腺素,同时在该条件下多巴、多巴胺不能形成发荧光的产物,不干扰它的测定,由此提出了一种灵敏、选择性测定肾上腺素的分析方法.
关键词： 三羟基吲哚法;  肾上腺素;  荧光光度法    
Abstract: An improved modification of the trihydroxyindole method was used to determine epinephrine in this paper. Experiments indicate that the fluorescent lutines can be stablized by the mercaptoethanol in the borax buffer(pH= 8. 7). So it is possible to determine epinephrine in the alkaline medium. Under the same conditions, dopa, dopamine can not form fluorescent products, therefore, they didn't interfere with the estimation of epinephrine. From this,a simple and selective method for the determination of epinephrine was put forward.
Key words: the trihydroxyindole method    epinephrine    spectrofluorimetry
出版日期: 2014-08-11
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童裳伦 1
吕国才 2
许志良 3

引用本文:

童裳伦 1,吕国才 2,许志良 3. 改进的三羟基吲哚法测定肾上腺素[J]. 浙江大学学报（理学版）, .

TONG Chang-lun 1,LU Guo-cai 2,XU Zhi-liang 3. Determination of epinephrine by an improved modification of the trihydroxyindole method.. Journal of Zhejiang University (Science Edition), .

链接本文:

https://www.zjujournals.com/sci/CN/    或    https://www.zjujournals.com/sci/CN/Y2000/V27/I5/530

[1] 朱京平;童裳伦;钮渭钧. Dy-BPMPHD一CTMAB荧光 体系的研究及其分析应用[J]. 浙江大学学报（理学版）, 1997, 24(2): 161-165.
[2] 沈报恩 王桂 良 彭 图 治 王 国顺 张 季 爽. 肾上腺素的薄层光谱电化学研究[J]. 浙江大学学报（理学版）, 1995, 22(1): 52-57.