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浙江大学学报（理学版）
浙江大学学报（理学版）
生命科学     
玻璃化冻存对胚胎神经细胞活性及脊髓移植的影响
胡军祥　姜玉新　周晴　郑斯涌　浙江大学西溪校区生命科学学院　杭州310012 曾跃武　冯春木　浙江大学西溪校区实验中心　杭州 310012
Effect of Vitrified Cryopreservation on Embryonic Neurocyte Viability and Transplantation for Spinal Cord Injury in Rats
Hu Junxiang　Jiang Yuxin　Zhou Qing　Zheng Siyong (College of Life Sciences of Zhejiang University, Hangzhou 310012) Zeng Yuewu Feng Chunmu (Central Laboratory of Zhejiang University, Hangzhou 310028)
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摘要： 采用玻璃化冻存技术对大鼠胚胎神经细胞进行深低温保存，在冻存1d、10d、20d和40d后，38℃水浴快速复温，离心洗脱冷冻保护剂，检测胚胎神经细胞的活性与功能，并移植于脊髓损伤大鼠.实验结果表明：玻璃化冻存40d的胚胎神经细胞，存活率为76.4±8.3％，膜完整性为冻存前的69.4±7.8％.细胞活力虽有下降，但仍可维持较高水平.培养1周的部分神经元建立突触联系，移植于脊髓损伤大鼠仍有一定的修复作用.
关键词： 胚胎神经细胞;  玻璃化;  脊髓移植    
Abstract: 　Rat embryonic cerebral neurocytes(RECN) were cryopreserved by vitrification at- 196℃. The samples were separately thawed after storage for 1、10、20 and 40 days,and then cryoprotective solution was diluted and removed. RECN were assayed for their viability and function,and were transplanted for spinal cord injury in rats. The results showed that RECN cryopreserved by vitrification for 40 days retained survival rate of 76.4±8.3％. Compared with the fresh neurocytes,the cell viabiliry and membrane integrity of the frozen-thawed RECN were decreased,but they still maintained at relatively high level.Synaptic connection was established in neuron culture for 1 week. Cryopreserved RECN were transplanted for spinal cord injury in rats, there was some recovery both in sensation and movement function. No obvious difference was found between vitrification and slow cooling group.
Key words: Embryonic cerebral neurocyte    Vitrification    Transplantation for spinal cord injury
收稿日期: 1998-05-22 出版日期: 2017-05-15
:  R318.52  
基金资助: 国家自然科学基金(No.39670241)和浙江省自然科学基金(No.397043)资助项目
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胡军祥，姜玉新，周晴，郑斯涌，曾跃武，冯春木. 玻璃化冻存对胚胎神经细胞活性及脊髓移植的影响[J]. 浙江大学学报（理学版）, .

Hu Junxiang，Jiang Yuxin，Zhou Qing，Zheng Siyong. Effect of Vitrified Cryopreservation on Embryonic Neurocyte Viability and Transplantation for Spinal Cord Injury in Rats. Journal of ZheJIang University(Science Edition), .

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http://www.zjujournals.com/sci/CN/    或    http://www.zjujournals.com/sci/CN/Y1999/V26/I1/65

[1] 夏阿根,徐芸,章林溪. 聚甲醛单链的玻璃化转变研究[J]. 浙江大学学报（理学版）, 2000, 27(6): 601-604.