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浙江大学学报（理学版）
浙江大学学报（理学版）
化学     
再生丝素固定葡萄糖脱氢酶的生物传感器
(嘉兴高等专科学校　嘉兴 314001) (杭州大学化学系　杭州 310028)
Biosensor of Glucose Dehydrogenase Immobilized by Regenerated Silk Fibroin
( Jiaxing College , Jiaxing 314001) (Department of Chemistry, Hangzhou University, Hangzhou 310028)
 全文: PDF(103 KB)  
摘要： 本文报导了以甲苯胺兰( TB)键合修饰浸蜡石墨电极为基体电极 , 用再生丝素将葡萄糖脱氢酶 (GDH) 、烟酰胺腺嘌呤二核苷(NAD+)固定在基体电极表面, 构成葡萄糖脱氢酶传感器. 在 pH=7. 6 的 NaOH-KH2PO4 介质中, 该传感器与葡萄糖浓度在 2. 0×10-5 ～ 5. 4×10-4mol·L -1范围内有良好的线性 关系, 响应时间为 20 s. 本文讨论了影响传感器响应电流的各种因素和测定葡萄糖的最佳条件. 用此传感 器测定了人血清中葡萄糖含量, 与酶法结果一致.
关键词： 再生丝素;  葡萄糖;  葡萄糖脱氢酶;  生物传感器     
Abstract: Anamperometric glucose dehydrogenase biosensor was reported.It is made up of Toluidine Blue(TB)modified graphite electrode of immerse wax and enzyme film of regenerated silk fibroin w as employed to immobilize glucose dehydrogenase( GDH)、nicotinamide adenine dinucleotide(NAD+). In pH=7. 6 NaOH-KH2PO4 medium, the biosensor has a linear response in the range of 2. 0×10-5～ 5. 4×10-4 mol·L-1 for concentration of glucose.The response time is 20s. The factors affcting the response current of sensor and optimal condition for the determination of glucose have been discussed. The sensor has been used to detect the concentration of glucose in human serum and the result conform to those obtained by enzyme analytical method.
Key words: regenerated silk fibroin    glucose    glucose dehydrogenase    biosensor
出版日期: 2017-12-06
CLC:  Q555  
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程　琼 习玲玲 施清照

引用本文:

程　琼 习玲玲 施清照. 再生丝素固定葡萄糖脱氢酶的生物传感器 [J]. 浙江大学学报（理学版）, .

Cheng Qiong　Xi Lingling　Shi Qingzhao　　. Biosensor of Glucose Dehydrogenase Immobilized by Regenerated Silk Fibroin . Journal of Zhejiang University (Science Edition), .

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https://www.zjujournals.com/sci/CN/    或    https://www.zjujournals.com/sci/CN/Y1998/V25/I4/63

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