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Radiological study on rat lumbar vertebral semidislocation model
CHEN Bo, LIN Xun, ZHANG Min, ZHAN Hong-sheng, SHI Yin-yu
Journal of ZheJiang University(Medical Science), 2015, 44(2): 117-123.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.001
Abstract( 351 )   HTML (   PDF(1748KB)( 654 )

Objective: To investigate the impact of fixation in part lumbar segment on rat model with radiography. Methods: One hundred and twenty SPF male SD rats (350~450g) were randomly divided into simple fixation group (SF group), rotary fixation group (RF group) and sham-operation group (Sham group). The external link fixation system was implanted into the L4-L6 of SF group and RF group rats. In RF group, L5 spinous process of rats was rotated to the right side, making the L4, L6 and L5 spinous process not in a straight line. In SF group, the external link fixation system was implanted simply and not rotated. The anterior and posterior diameter in the intervertebral space and the distance between spinous processes were examined 1, 4, 8 and 12 weeks after fixation.Results: The anterior diameter in the intervertebral space between L3-L4, L4-L5, L5-L6, and L6-S1 of SF groups 4 weeks after fixation were 28.11±3.94, 25.73±4.70, 29.51±6.34 and 34.97±4.65 pixels; the posterior diameter were 7.39±1.63, 6.65±1.76, 7.02±1.52 and 9.62±2.50 pixels; the distance between spinous process were 39.33±11.74, 14.11±5.75, 21.32±6.84 and 77.43±13.69 pixels, the values were less than those in Sham groups (P<0.05). The anterior and posterior diameter in the intervertebral space and the distance between spinous process of SF groups 8 and 12 weeks after fixation continuously decreased compared with Sham group rats (P<0.05). The results of RF groups were consistent with the measurement of SF groups, the anterior and posterior diameter in the intervertebral space and the distance between spinous process of RF group rats were less than those in Sham groups 4, 8 and 12 weeks after fixation (P<0.05). Osteophyte formation was observed in SF and RF groups 8 weeks after fixation. Conclusion: The fixation in part lumbar segment can result in the decrease of intervertebral space and the formation of osteophytes.

Effect of spinal manipulation on brain functional activity in patients with lumbar disc herniation
YUAN Wei-an, SHEN Zhi-bi, XUE Li, TAN Wen-li, CHENG Ying-wu, ZHAN Song-hua, ZHAN Hong-sheng
Journal of ZheJiang University(Medical Science), 2015, 44(2): 124-130,137.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.002
Abstract( 351 )   HTML (   PDF(4172KB)( 360 )

Objective: To investigate the effects of spinal manipulation (SM) on brain functional activity in patients with lumbar disc herniation (LDH).Methods: Eleven patients with LDH were recruited in the study. All patients received 6 times of lumbar SM treatment and then clinical efficacy was evaluated. All patients received brain functional magnetic resonance imaging (fMRI) scans before and after SM treatment. Results: Three subjects dropped out and 8 subjects completed the study, among whom 4 cases were effective and 4 ineffective after SM treatment. The required pressure value producing the same level (VAS 50) pain was (7.43±1.47) kg and (10.53±0.55) kg before and after SM treatment in effective patients(P<0.05); however, there was no significant difference in ineffective patients (P>0.05). Compared to pre-treatment level, the brain functional activity in effective patients was mainly inhibited, the inhibited areas were located in the right side of prefrontal cortex and cerebellum; while the brain functional activity was generally enhanced in ineffective patients. Conclusion: SM can affect the brain functional activity of patients with LDH, the inhibited areas is mainly located in prefrontal cortex and cerebellum when SM treatment is effective.

Construction of a human cervical spine with bilateral vertebral artery fluid-solid coupling model
WANG Hui-hao, SHEN Zhi-bi, DENG Zhen, WANG Kuan, ZHAN Hong-sheng
Journal of ZheJiang University(Medical Science), 2015, 44(2): 131-137.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.003
Abstract( 312 )   HTML (   PDF(1526KB)( 232 )

Objective: To construct a human cervical spine with bilateral vertebral artery fluid-solid coupling model. Methods: Helical CT images under the principle of reverse engineering and meshed in finite element model(FEM) related software were used to establish a human cervical spine with bilateral vertebral artery fluid-solid coupling model. In the process of modeling of vertebral body, vertebral artery, ligament, intervertebral disc, cartilage and endplate large anatomic data and cadaver experiments results were referenced. From the morphology and function the simulation of model with real physiological status was tested. Results: The study showed that the stress concentration on the surface of vertebral body and the blood wall of the bilateral vertebral artery, and the result of the volume flow rate-time curve of bilateral vertebral artery of the model were consistent with the published literatures. This model was well consistent with the clinical phenomenon. Conclusion: The three-dimensional FEM of the human cervical spine established by the introduced method has been effectively verified. The modeling method would provide a new tool for research on the cervical spine biomechanics.

Development of Chinese version vertigo symptom scale(VSS):reliability and validity
DENG Zhen, YUAN Wei-an, WANG Hui-hao, ZHAN Hong-sheng
Journal of ZheJiang University(Medical Science), 2015, 44(2): 138-144.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.004
Abstract( 430 )   HTML (   PDF(653KB)( 306 )

Objective: To develop a Chinese version of Vertigo Symptom Scale (VSS-C) and to examine its reliability and validity. Methods: The VSS was translated into Chinese and developed a Chinese version VSS (VSS-C) with the consent of the author. The VSS-C scale was tested in 52 subjects with cervical spondylosis of vertebral artery type(CSA group)and 21 healthy subjects (control group). In CSA group VSS-C scale and SF-36 scale investigation was performed for 2 times with 1 week interval, after receiving 1-week orthopedic rehabilitation the patients were evaluated with the VSS-C and SF-36 scale; while subjects in control group received the investigation twice in two weeks. The reliability of the scale was evaluated with Cronbach's alpha method and the correlation between SF-36 scale and the VSS-C were also evaluated for the validity. Results: The internal consistency of VSS-C was good with Cronbach's alpha of 0.886. Test-retest reliability was also very good with an intraclass correlation coefficient (ICC) between two time points, being 0.970 for VSS-C,0.965 for VSS-AA and 0.992 for the VSS-VER. Regarding concurrent validity,significant low correlation was found between the VSS-AA and VSS-VER (r=0.379,P<0.05). Significant expected correlation was detected between the VSS-C and SF-36 (r>0.5,P<0.05). The independent-samples t test results (t=6.261,P<0.01) of the CSA group and the control group showed that the VSS-C was able to distinguish healthy people from CSA patients. The paired-samples t test results (t=5.513,P<0.01) showed that VSS-C reflected the improvement of patients after treatment of Chinese massage manipulation. Conclusion: The Chinese version of VSS has a high comprehensibility, internal consistency and validity, and it can be a useful instrument for evaluation of patients with cervical spondylosis of vertebral artery type in China.

Screening of active compounds with myocardial protective effects from Tongmai Yangxin pill
TAO Shan, LIANG Xin-ying, WANG Yi, WANG Yi
Journal of ZheJiang University(Medical Science), 2015, 44(2): 145-153.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.005
Abstract( 365 )   HTML (   PDF(1843KB)( 347 )

Objective: Based on cell model and HPLC-MS technology, to screen myocardial protection active compounds from traditional patent medicine Tongmai Yangxin pill (TMYXP). Methods: Fractions of TMYXP were prepared by high performance liquid preparation technology. The cardioprotective effects of prepared fractions were tested on H2O2 oxidation-damaged H9c2 myocardiocytes. The active components were analyzed by high performance liquid chromatography (HPLC) coupled with high resolution mass spectrometry. The possible active compounds were putatively identified by comparison of their MS ions and molecular weight with literatures. Results: Ten TMYXP components presented significant myocardial protective activities, 5 of which were investigated and presented good dose-effect relationships. Their median effective concentrations (EC50) were respectively 11.66, 17.44, 13.10, 7.332, 15.15 μg/mL. Totally, 11 potential active compounds were analyzed and identified, including Glycyrrhizic acid, Glycycoumarin, Licoisoflavone, Ophiopogonin D', Licoricon, Gancaonin L, Neoglycyrol, Emodin, Angeloylgomisin H, Angeloylgomisin Q and Glyasperin A.Conclusion: The myocardial protection active compounds of TMYXP were screened successfully.

Th17 and Treg cell levels in patients with sarcoidosis and their relation to disease activation
WENG Yue-song, WANG Hua-ying, LV Ding-feng, FU Zhong-ming, YU Wan-jun
Journal of ZheJiang University(Medical Science), 2015, 44(2): 154-161.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.006
Abstract( 292 )   HTML (   PDF(2484KB)( 258 )

Objective: To investigate the Th17 cell and Treg cell levels in patients with sarcoidosis, and their relation to disease activation and glucocorticoids treatment. Methods: Twenty-three sarcoidosis patients admitted in Yinzhou People's Hospital from January 2009 to December 2013 and 25 healthy subjects (controls) were included in this study. The blood samples and bronchoalveolar lavage fluid (BALF) samples were collected in all patients before and after glucocorticoids treatment. The serum angiotensin converting enzyme (SACE) levels were detected. The percentages of Th17 cells and Treg cells in peripheral blood and BALF were determined by flow cytometry, the concentrations of cytokines in serum and supernatants of BALF were measured by enzyme-linked immunosorbent assay (ELISA). The levels of ROR-γt and Foxp3 mRNA transcripts in peripheral blood mononuclear cells (PBMC) were determined by real-time quantitative PCR. The potential correlation between the percentages of Th17 or Treg cells and SACE levels was evaluated. Results: Compared with healthy controls, significantly higher frequencies of Th17 cells (4.34%±0.89% vs 1.60%±0.42%), lower frequencies of Treg cells (1.28%±0.37% vs 3.39%±0.50%) in peripheral blood were observed. Higher level of ROR-γt mRNA (21.31±3.55 vs 3.63±1.00) and lower level of Foxp3 mRNA (1.60±0.24 vs 3.12±0.76) in peripheral blood were detected in sarcoidosis patients in active stage (before glucocorticoids treatment) (all P<0.01). After the treatment of glucocorticoids, these index in peripheral blood were significantly improved (Th17 cells 2.16%±0.68%,Treg cells 2.21%±0.42%, ROR-γt mRNA 10.15±1.93, Foxp3 mRNA 2.44±0.38)(all P<0.05). The changing trends of Th17 and Treg cell cytokines levels in serum were consistent with two type cells. Meanwhile, the changing trends of above index in BALF of patients treated by glucocorticoids were consistent with those in sarcoidosis patients in active stage. The increased ratios of Th17 cells to Treg cells were positively correlated with the level of serum SACE (r= 0.781). Conclusion: The imbalance of Th17 cells and Treg cells in peripheral blood and airway may be involved in the pathogenesis of sarcoidosis, which was associated with the activity of disease, and the treatment of glucocorticoids may achieve a therapeutic effect by correcting the immune imbalance.

Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-catenin signaling pathway in mesenchymal stem cells
ZHANG Chun-yang, ZHU Yan, FENG Hua-song, CHEN Xu-xin
Journal of ZheJiang University(Medical Science), 2015, 44(2): 162-166.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.007
Abstract( 322 )   HTML (   PDF(1063KB)( 306 )

Objective: To investigate the effect of irradiated human lung fibroblasts (HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs). Methods: HUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays (co-culture group). The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture. WISP-1 protein levels in conditioned medium were examined by ELISA. Results: The levels of p-GSK3β/GSK3β(0.15±0.05), FRAT1(0.48±0.07) and β-catenin(0.50±0.07)in co-cultured HUMSCs significantly decreased compared to those in single group (0.55±0.05, 1.16±0.13 and 2.39±0.15, all P<0.05).The supernatant level of WISP-1 in co-culture group was significantly decreased[(602.23±161.47)ng/mL], compared to the single group[(977.77±110.56)ng/mL, P<0.05].Conclusion: Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro.

Preretinal hemorrhage and prognosis following vitrectomy and silicone oil tamponade for severe proliferative diabetic retinopathy
LI Jiu-ke, JIN Xiao-hong, FANG Wei, FENG Li-guo, ZHAI Jing, LI Yu-min
Journal of ZheJiang University(Medical Science), 2015, 44(2): 167-173.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.008
Abstract( 448 )   HTML (   PDF(1862KB)( 275 )

Objective: To examine the prognosis of preretinal hemorrhage following vitrectomy and silicone oil tamponade for severe proliferative diabetic retinopathy.Methods: Clinical data of 76 cases of proliferative diabetic retinopathy treated with vitrectomy and silicone oil infusion tamponade in Sir Run Run Shaw Hospital from October 2006 to September 2013 were retrospectively reviewed. Intraoperative bleeding,postoperative preretinal bleeding,blood reabsorption time, and preretinal fibrosis were assessed.Results: All preretinal hemorrhage developed within 1 week after surgery, blood was distributed in thin and scattered patterns (32 cases), thick and localized patterns (25 cases) or thick and scattered patterns (19 cases). The preretinal hemorrhage was ceased in 1 day after operation in 35 cases, in 2 days after operation in 18 cases, in two weeks after operation in 23 case. Recurrent hemorrhage occurred within 1 week after operation in 15 cases. Thin blood was largely reabsorbed in about two weeks, and thick blood was largely reabsorbed in about five weeks. Fibrosis tissue was resulted in 15 cases(34.1%) with thick blood. Conclusion: Most of preretinal hemorrhage occurs within 1 week after surgery and is reabsorpted with 5 weeks in patients with proliferative diabetic retinopathy undergoing vitrectomy and silicone oil tamponade. The major complication of preretinal bleeding is the formation of preretinal fibrosis.

Apoptosis of acute myeloid leukemia HL-60 cells induced by CDK inhibitor SNS-032 and its molecular mechanisms
HAN Yan-xia, YOU Liang-shun, LIU Hui, MAO Li-ping, YE Xiu-jin, QIAN Wen-bin
Journal of ZheJiang University(Medical Science), 2015, 44(2): 174-178.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.009
Abstract( 342 )   HTML (   PDF(740KB)( 346 )

Objective: To investigate the effects of cycle-dependent kinase (CDK) inhibitor SNS-032 on apoptosis in human acute myeloid leukemia (AML) HL-60 cells and its molecular mechanisms. Methods: Cultured AML HL-60 cells were treated with various concentrations of SNS-032. Cell apoptosis was determined with flow cytometry;cell viability was measured by MTT assay; the profiles of microRNA expression of HL-60 cells were analyzed by microRNA microarray;the protein expressions of JAK2/STAT3 pathway were detected by Western blotting. Results: Apoptosis of AML HL-60 cells was induced by SNS-032; the rate of apoptosis was (5.9±1.7)%, (12.1±3.1)% and (59.4±3.6)% when HL-60 cells were treated with 0,100 and 200 nmol/L SNS-032. MicroRNA microarray analysis revealed that the levels of miR-30a, miR-183, miR-20b, miR-26b, miR-20a, miR-589, miR-107, miR-181a, miR-106a, miR-17 and miR-378c were down-regulated by SNS-032,whereas the levels of miR-320a and miR-H7* were up-regulated. Western blotting showed that SNS-032 strongly inhibited phosphorylation of STAT3 and protein expression of JAK2,C-MYC and MCL-1. Conclusion: CDK inhibitor SNS-032 can induce apoptosis of AML HL-60 cells, which is associated with the inhibition of MCL-1,C-MYC and JAK2/STAT3, and down-regulation of miR-17-92 family.

Imatinib mesylate for chronic myeloid leukemia:in patients with initial treatment versus those with Recombinant Human IFN-α2b treatment failure
JIANG Jin-hong, XU Wei-lai, QIAN Wen-bin
Journal of ZheJiang University(Medical Science), 2015, 44(2): 179-183.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.010
Abstract( 257 )   HTML (   PDF(477KB)( 264 )

Objective: To evaluate the efficacy of imatinib mesylate (IM) for patients with newly diagnosed chronic myeloid leukemia (CML) and patients after failure of Recombinant Human interferon-α2b (IFN-α2b) therapy. Methods: A total of 86 patients with CML in chronic-phase, including 61 newly diagnosed cases and 25 cases of IFN-α2b failure, who received IM at 400 mg daily were retrospectively analyzed. Conventional cytogenetic analysis of R-banding was used to detect chromosome abnormalities and real-time PCR was used to detect BCR-ABL fusion gene.Results: 81.9% of newly diagnosed patients and 36.0% of IFN-α2b failure patients achieved partial cytogenetic response (PCyR) by 6 months. In addition, 86.9% of newly diagnosed patients and 68.0% of IFN-α2b failure patients achieved complete cytogenetic response (CCyR) in 24 months. There was significant difference between two groups (P<0.001). The median time achieved CCyR in newly diagnosed group and IFN-α2b failure group were 6 months and 15 months, respectively. Compared with newly diagnosed group, IFN-α2b failure group showed lower rate of complete molecular remission (CMR) (70.4%vs 40.0%, P=0.033). There are 14 patients (22.9%) in newly diagnosed patients with cytogenetic resistance, among whom 4 with primary cytogenetic resistance; while there were 14 patients (56.0%) in IFN-α2b failure group with cytogenetic resistance, all of whom with primary resistance. Conclusion: Compared with newly diagnosed patients, CML patients after failure of IFN-α2b therapy have a high rate of primary cytogenetic resistance and low response rate to IM.

Expression of neuron-specific enolase and synaptophysin in esophageal development of human embryos
LIU Xue-hong, ZHANG Yong, LIU Chuan-kang
Journal of ZheJiang University(Medical Science), 2015, 44(2): 184-188.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.011
Abstract( 324 )   HTML (   PDF(1843KB)( 250 )

Objective: To investigate the expression of neuron-specific enolase (NSE) and synaptophysin(SYN) proteins in different developmental stages of human embryonic esophagus. Methods: Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age (n=16). One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age. Results: In fetuses with 2, 3 and 4 months of gestation, the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38±8.37, 25.00±11.54 and 38.00±15.09, respectively; the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38±14.93, 62.25±18.59 and 56.44±14.70, respectively. NSE-positive cells were detected in the esophageal epithelium only at the third month. In the fetuses at 2, 3 and 4 months of gestation, SYN in all layers of esophageal tissue were positively or strong positively expressed, especially in the myenteric plexus and submucosal plexus. The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2, 3 and 4 month gestation were 54.69±9.34, 51.84±6.10 and 46.41±6.44, respectively. Conclusion: SYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development.

Body mass index and all-cause mortality in Asian adults:a meta-analysis
WANG You-qing, YE Ding, LI Ying-jun, CHEN Kun
Journal of ZheJiang University(Medical Science), 2015, 44(2): 189-196.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.012
Abstract( 295 )   HTML (   PDF(1096KB)( 346 )

Objective: To perform a systematic review between all-cause mortality and body mass index (BMI) in Asian adults. Method:Relevant prospective studies that reported the relative risks (RRs) of all-cause mortality for community-based adults in Asia were identified by a literature search.PubMed and CNKI electronic databases were searched from inception through September 30, 2014, with language restrictions of English and Chinese. Data were extracted by 1 reviewer and then reviewed by 3 independent reviewers. The overall effect of varied levels of BMI and all-cause mortality were then pooled and analyzed.Potential sources of heterogeneity were detected by stratification analyses and sensitivity analyses. Publication bias was detected by funnel plot, Egger's test and Begg's test. Results:Seventeen studies met the inclusion criteria; these studies included 1 769 369 individuals with 104 888 deaths. Random-effects summary all-cause mortality RRs was calculated. With the use of a BMI (in kg/m2) of 18.5~22.9 as the reference, the summary RRs were 1.39(95%CI:1.31~1.47) for BMI less than 18.5 kg/m2, 0.88 (95%CI:0.85~0.92) for BMI of 23.0~29.9 kg/m2 and 1.14 (95%CI:1.05~1.23) for BMI more than 30.0 kg/m2. The RRs tended to be higher when weight and height were self-reported rather than measured. The RRs were higher when papers were published before the year 2005 rather than after the year 2006. Also, the RRs were higher when the quality scores were higher. Potential sources of heterogeneity were gender, the method of obtaining weight and height, geography, publication year and quality scores. There was no publication bias(P>0.05) in this meta-analysis. Conclusion: There was an increased risk of all-cause mortality for those both at the lower and higher level of BMI in Asian adults.

Effects of DNMT3A gene mutations on prognosis of patients with acute myeloid leukemia:a meta-analysis
XI Xiao-ping, ZENG Ling-xia, Yu Fang-fang, LIU Hua-sheng
Journal of ZheJiang University(Medical Science), 2015, 44(2): 197-203.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.013
Abstract( 512 )   HTML (   PDF(865KB)( 335 )

Objective: To evaluate the effects of DNMT3A gene mutation on prognosis of patients with acute myeloid leukemia (AML) by a meta-analysis.Methods: Methods of Cochrane systematic review was followed by 7 databases,including PubMed, Embase, Ovid, CNKI, CBM, WanFang Data and VIP, were searched for peer-reviewed articles related to DNMT3A gene mutations and prognosis of patients with AML.Then manual retrieval was applied into literature references. After the evaluation of quality and extract of clinical trialliterature data, Stata 11.0 was employed to perform meta-analysis.Results: Seven randomized controlled trials involving 1493 cases were included in the meta-analysis. The prognosis of patients with DNMT3A mutations and without DNMT3A mutations was compared. There was no statistically significant difference in complete remission(CR) rate (OR=1.034, 95%CI:0.596~1.796, P=0.905)between two groups, but the overall survival (OS)(HR=1.990, 95%CI:1.463~2.510, P=0.000)and disease free survival (DFS)(HR= 2.840, 95%CI :1.063~4.613, P=0.002,)of patients without DNMT3A mutations were longer than those with DNMT3A mutation.Conclusion: DNMT3A gene mutation is an independent risk factor of poor prognosis of patients with acute myeloid leukemia.

Progress on association between autophagy and cancer
CAO Jian-ping, XIA Da-jing
Journal of ZheJiang University(Medical Science), 2015, 44(2): 204-210.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.014
Abstract( 341 )   HTML (   PDF(668KB)( 283 )

Autophagy is an evolutionarily well conserved cellular catabolic process.Cellular proteins and organelles are engulfed to autophagosomes and eventually delivered to lysosomes for degradation. Autophagy is closely associated with a variety of human diseases especially cancer. At present, it has been widely accepted that autophagy is a “double-edged sword” in cancer:it blocks tumorigenesis at the early stage, while it facilitates tumor development at the promotion and progression stage. Moreover, autophagy can be induced by chemotherapy and radiotherapy, which is generally play a pro-survival function. Thus, autophagy is an important target for cancer therapy, and suppression of autophagy to enhance the efficacy of cancer therapeutic agents is a novel strategy in cancer therapy under active clinical trials.

Research progress on epithelial-mesenchymal transition in cancer recurrence and metastasis
LIU Zhi-xian, WEI Er-qing, LU Yun-bi
Journal of ZheJiang University(Medical Science), 2015, 44(2): 211-216.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.015
Abstract( 397 )   HTML (   PDF(544KB)( 435 )

Epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their morphology and function and gradually transformed into mesenchymal-like cells. It is considered that EMT is the main cause for tumor recurrence and metastasis. Many factors are involved in the regulation of EMT, such as E-cadherin, transforming growth factor-β, Wnt signaling pathway, microRNA and EMT-related transcription factors. This article reviews the research progress on EMT and the involved mechanisms, and thus to provide a new perspective on cancer therapy in the future.

Research advances on ubiquitin C-terminal hydrolase in oncogenesis and progression
YU Juan, CHEN Wei-lin
Journal of ZheJiang University(Medical Science), 2015, 44(2): 217-222.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.016
Abstract( 260 )   HTML (   PDF(561KB)( 265 )

By regulating the ubiquitination and deubiquitination of key proteins, ubiquitin-proteasome system mediates a variety of cellular activities. Ubiquitin C-terminal hydrolase (UCH) is a deubiquitinating enzyme which can remove ubiquitin chains at the end of ubiquited proteins. The abnormal expression of UCH has been found in a variety of tumor tissues, indicating that it participates in the process of tumor development. Here we review the characteristics of UCH members and current understanding about the role of UCH in tumor development, and the potential target for cancer treatment.

Progress on interleukin-27 in tumor immunity
HE Jia, WANG Qing-qing
Journal of ZheJiang University(Medical Science), 2015, 44(2): 223-228.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.017
Abstract( 451 )   HTML (   PDF(494KB)( 313 )

As a member of IL-6/IL-12 cytokine family, IL-27 is a heterodimeric cytokine composed of the p28 and EBI3. Functioning as a linkage between innate and adaptive immunity, IL-27 is mainly produced by activated antigen-presenting cells and has a variety of responder cells including T cells, NK cells, macrophages and dendritic cells. IL-27 plays an antitumor role through promoting the production of cytotoxic T cells, regulating the differentiation of T cell subsets, enhancing the function of NK cells and inhibiting the angiogenesis. On the other hand, IL-27 may also have a positive effect on tumor progress and metastasis. IL-27 can be used as a reference marker for tumor diagnosis, due to its relation to the occurrence and development of tumors. This article reviews the research progress on the roles of IL-27 in tumor biological regulation.

Carotid artery dissection with Horner syndrome as main manifestation:a case report
FANG Xing, XU Zi-qi, LUO Ben-yan, YUAN Huai-wu, ZHU Xiong-chao, YUAN Yuan
Journal of ZheJiang University(Medical Science), 2015, 44(2): 229-232.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.018
Abstract( 314 )   HTML (   PDF(1958KB)( 278 )

A 44-year old male patient was admitted to the First Affiliated Hospital,Zhejiang University School of Medicine with left ptosis and pain on the left head and neck for 20 days.Brain MRI showed subacute cerebral infarction on left parietal lobe and intramural hematoma on left internal carotid artery. CT angiography showed stenosis line on the C1 segment of left internal carotid artery. Digital subtraction angiography showed dissection on the C1 segment of left internal carotid artery.The condition of patients was improved after anticoagulant therapy.

Pure spinal epidural cavernous hemangioma:report of one case
HE Zhan, DING Fang, RONG Jian-dong, ZHU Guang-yao
Journal of ZheJiang University(Medical Science), 2015, 44(2): 233-235.   https://doi.org/10.3785/j.issn.1008-9292.2015.03.019
Abstract( 319 )   HTML (   PDF(1134KB)( 231 )

A 55-year-old man presented with progressive numbness and weakness of both lower limbs is reported. MRI demonstrated a pure epidural lesion at T3-6 space appearing as isointense on T1-weighted images with enhancement by contrast medium and hyperintense on T2-weighted images. The lesion was totally removed microsurgically. Histological examination revealed cavernous hemangioma. The patient made a good recovery after surgery.

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