J Zhejiang Univ (Med Sci)  2019, Vol. 48 Issue (5): 526-532    DOI: 10.3785/j.issn.1008-9292.2019.10.10
Effect of bilateral superior cervical sympathetic ganglion occlusion on pathological process of aortic dissection and its mechanism
ZHANG Zhenjun1(),WANG Hu2,*(),ZHANG Yujing3,SU Jinlin2,LI Jianjun2
1. Department of Cardiology, General Hospital of Ningxia Medical University, Yinchuan 750000, China
2. Department of Cardiothoracic Surgery, General Hospital of Ningxia Medical University, Yinchuan 750000, China
3. Department of Cardiac and Vascular Surgery, General Hospital of Ningxia Medical University, Yinchuan 750000, China

Abstract

Objective: To investigate the effect of bilateral superior cervical sympathetic ganglion occlusion (SCG) on aortic dissection and its possible mechanism. Methods: Forty-five SD rats were randomly divided into three groups with 15 in each:blank control group, sham operation group and SCG group. β-aminopropione (666 mg·kg-1·d-1) was given by subcutaneous injection for 4 weeks to establish the aortic dissection model. Rats in SCG group were given SCG before the injection of β-aminopropione. Blood pressure and heart rate of the rats were monitored using noninvasive tail artery blood pressure measuring instrument; sympathetic activity was monitored using drug block method; the structure of aortic wall was observed using HE staining; collagen fibers in aortic wall was observed using Sirius red staining; protein expression of Apelin was detected by immunohistochemistry; and the protein expression of matrix metalloproteinase (MMP)-2, 9 was detected by Western blotting. Results: During the experiment, the body mass of the sham operation group and SCG group was smaller than that of the blank control group (all P < 0.05), and the body mass of the SCG group was larger than that of the sham operation group (all P < 0.05). The heart rate and sympathetic activity of the sham operation group were higher than those of the blank control group (all P < 0.05), while the SCG group were lower (all P < 0.05). Compared with the blank control group, the aortic wall in the sham operation group was thickening, while that in the SCG group was improved. A large number of collagen-1 in the aortic wall of the blank control group was stained brown by Sirius red, which was lighter in SCG group, and the staining in the sham operation group was the lightest. Compared with the blank control group, the expression of Apelin, MMP-2 and MMP-9 protein in the sham operation group increased (all P < 0.05), while those in the SCG group decreased (all P < 0.05). Conclusion: SCG can effectively reduce the incidence and mortality of aortic dissection in rats, which may be related to the inhibition of sympathetic activity and the decrease of collagen-1, Apelin, MMP-2 and MMP-9 expression.

Received: 30 April 2019      Published: 04 January 2020
 CLC: R361+.1
Corresponding Authors: WANG Hu     E-mail: 601948902@qq.com;wangfirstday@163.com
 Fig 1 Structural changes of arterial wall in three groups (HE staining) Fig 2 Body weight changes during the experiment Tab 1 Blood pressure, heart rate and sympathetic nerve activity among three groups (${\bar x}$±s) Fig 3 Aortic vascular wall thickness and collagen 1 content in three groups Fig 4 Apelin protein expression in aorta of three groups Fig 5 Electrophoresis of MMP-2, MMP-9 protein in aortic tissue in three groups Tab 2 Relative expression of MMP-2 and MMP-9 in three groups (${\bar x}$±s)