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J Zhejiang Univ (Med Sci)  2018, Vol. 47 Issue (6): 665-670    DOI: 10.3785/j.issn.1008-9292.2018.12.16
    
Research progress on composite animal models of inflammatory bowel disease based on gene knockout
ZHAO Huihui(),TANG Huifang*()
Zhejiang University School of Medicine, Hangzhou 310058, China
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Abstract  

Establishing a suitable animal model is important for studying the mechanism of inflammatory bowel disease (IBD) and exploring new therapeutic approaches. Although a large number of IBD single gene knockout animal models have been established, single knockout of certain genes associated with human IBD susceptibility does not manifest symptoms of IBD or manifest extremely milder symptoms, while composite animal models based on other modeling factors can better simulate the clinical features of IBD. This article mainly introduces three novel composite animal models and elaborates the possible pathogenesis of each composite model:animal models established by gene double knockout have more obvious and earlier symptoms than single-knockout models; single gene knockout model with Helicobacter infection can help to study the role of microbial infections in the pathogenesis of IBD; on the basis of gene knockout, specific deletion of certain immune cells can be used to study the role of the immune cells in the development of IBD. Among the above composite animal models, Muc2/IL-10 double knockout mice may be important animal models for IBD study.



Key wordsInflammatory bowel diseases      Mice, knockout      Disease models, animal      Review     
Received: 10 April 2018      Published: 15 March 2019
CLC:  R574  
Corresponding Authors: TANG Huifang     E-mail: 3140104256@zju.edu.cn;tanghuifang@zju.edu.cn
Cite this article:

ZHAO Huihui,TANG Huifang. Research progress on composite animal models of inflammatory bowel disease based on gene knockout. J Zhejiang Univ (Med Sci), 2018, 47(6): 665-670.

URL:

http://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2018.12.16     OR     http://www.zjujournals.com/med/Y2018/V47/I6/665


基于基因敲除的炎性肠疾病复合动物模型研究进展

建立合适的动物模型对于研究炎症性肠疾病(IBD)的机制以及探索新的治疗途径具有重要意义。单敲除某些与人类IBD易感性相关的基因并不表现为IBD的症状或症状较轻,而结合其他造模因素建立的复合动物模型能更好地模拟IBD的临床特征。本文主要介绍了三类新型复合动物模型的具体特点:基因双重敲除动物模型较单基因敲除模型的建立周期更短、疾病症状更明显;螺杆菌复合基因敲除模型有助于研究微生物感染在IBD发病机制中的作用;在基因敲除的基础上特异性缺失某种免疫细胞可用于研究该免疫细胞在IBD发展中的作用。这些模型在一定程度上有助于探索IBD的机制,其中Muc2/IL-10双重敲除模型有望成为IBD遗传学研究的重要动物模型。


关键词: 炎性肠疾病,  小鼠, 基因敲除,  疾病模型, 动物,  综述 
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