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Journal of ZheJiang University(Medical Science)  2014, Vol. 43 Issue (2): 141-144    DOI: 10.3785/j.issn.1008-9292.2014.03.003
    
Quantitative determination of voglibose contents in its tablets with high-performance liquid chromatography-mass spectrometry
ZHANG Xiang-qun, WU Ling-hua,CHEN Fang-jun, ZHANG Yi, CHEN Lu
Chiatai Qingchunbao Pharmaceutical Co. Ltd, Hangzhou 310023, China
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Abstract  

Objective: To develop a method for determination of voglibose contents in its tablets by high-performance liquid chromatography-mass spectrometry (HPLC-MS). Methods: The measurements were carried out on an Agilent ZORBAX Eclipse Plus C18 column (2.1×150mm 3.2μm) with a temperature of 40℃. A mixture of methanol and water (2∶3, v/v) was used as a mobile phase at a flow rate of 0.25 ml/min. Voglibose was detected in an electrospray ionization (ESI) mode with MRM. Results: The calibration curves of voglibose showed good linearity in a range of 1.5804-2.6340 μg/ml (r=0.9990). The average recovery was 100.2% with RSD of 1.37% (n=6) for m/z 268.2/74.2. Linearity was obtained with r=0.9976 and the average recovery was 99.3% with RSD of 1.78% (n=6) for m/z 268.2/92.2. Conclusion: HPLC-MS method is accurate, reproducible and can be used for quality control of voglibose tablets.



Key wordsInositol/analysis      Inositol/analogs &      derivatives      Retrieving rate      Temperature      Chromatography, high pressure liquid      Mass spectrometry      Voglibose     
Received: 09 December 2013     
Corresponding Authors: WU Ling-hua, E-mail: 179680521@qq.com   
Cite this article:

ZHANG Xiang-qun, WU Ling-hua,CHEN Fang-jun, ZHANG Yi, CHEN Lu. Quantitative determination of voglibose contents in its tablets with high-performance liquid chromatography-mass spectrometry. Journal of ZheJiang University(Medical Science), 2014, 43(2): 141-144.

URL:

http://www.zjujournals.com/xueshu/med/10.3785/j.issn.1008-9292.2014.03.003     OR     http://www.zjujournals.com/xueshu/med/Y2014/V43/I2/141


高效液相色谱-质谱法测定伏格列波糖片中伏格列波糖含量

目的:建立高效液相色谱-质谱法测定伏格列波糖片中伏格列波糖的含量。方法:采用Agilent ZORBAX Eclipse Plus C18(2.1 mm×150 mm, 3.2 μm)色谱柱,柱温40 ℃,流速为0.25 ml/min,甲醇-水(2∶3)为流动相,流速为0.25 ml/min,质谱检测中采用电喷雾离子源多反应检测扫描(MRM)模式。结果:伏格列波糖浓度在1.5804~2.6340 μg/ml范围内线性关系良好,其中离子对268.2/74.2(r=0.9990)平均回收率为100.2%,相对标准偏差为1.37%(n=6);离子对268.2/92.2(r=0.9976)平均回收率为99.3%,相对标准偏差为1.78%(n=6)。结论:高效液相色谱-质谱法准确性、重复性均好,可用于伏格列波糖片的质量控制。


关键词: 肌醇/分析,  肌醇/类似物和衍生物,  回收率,  温度,  色谱法,  高压液相,  质谱分析法,  伏格列波糖 
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