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Journal of ZheJiang University(Medical Science)  2013, Vol. 42 Issue (1): 52-    DOI:
    
Expression and distribution of cysteinyl leukotriene receptors CysLT1R and CysLT2R,and GPR17 in brain of Parkinson disease model mice
WANG Hao1,2,SHI Qiaojuan2,SHI Wenzhen2,ZHANG Xiayan2,WANG Xiaorong2,ZHANG Lihui3,FANG Sanhua2,LU Yunbi2,ZHANG Weiping2,WEI Erqing2
1.Department of Neurology,Tongde Hospital of Zhejiang Province,Hangzhou 310012,China;  2.Department of Pharmacology,Zhejiang University School of Medicine,Hangzhou 310058,China;  3.Department of Pharmacology,School of Medicine,Hangzhou Normal University,Hangzhou 310036,China
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Abstract  Objective: To examine the spatiotemporal profiles and localization of CysLT1R,CysLT2R and GPR17 in mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson disease (PD).
Methods: PD model was induced by subcutaneous injection of MPTP (25 mg/kg) for 5 d in adult male C57BL/6 mice.At d10 after MPTP injection,the expression and cellular localization of CysLT1R,CysLT2R and GPR17 in the substantia nigra were detected by immunohistochemistry and immunofluorescence.
Results: CysLT1R,CysLT2R and GPR17 were normally localized in TH-positive dopaminergic neurons and microglia,while CysLT2R was also expressed in astrocytes.In dopaminergic neurons,approximately 91% co-expressed GPR17,77% co-expressed CysLT1R and 52% co-expressed CysLT2R.Compared with the control group,TH-positive cells in the substantia nigra were significantly reduced in PD mice.CysLT1R,CysLT2R and GPR17-positive cells were significantly reduced; and CysLT1R,CysLT2R,GPR17-positive dopaminergic neurons were also significantly reduced in the PD group.In the striatum,both CysLT1R and GPR17 were normally expressed in neurons; whereas CysLT2R was expressed in astrocytes.In PD striatum,CysLT1R and GPR17-positive cells were decreased,but CysLT2R expression was significantly increased which mainly expressed in the proliferating astrocytes.
Conclusion: CysLT1R,CysLT2R and GPR17 may be involved in the MPTP-induced PD damage in mice.


Cite this article:

. Expression and distribution of cysteinyl leukotriene receptors CysLT1R and CysLT2R,and GPR17 in brain of Parkinson disease model mice. Journal of ZheJiang University(Medical Science), 2013, 42(1): 52-.

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http://www.zjujournals.com/xueshu/med/     OR     http://www.zjujournals.com/xueshu/med/Y2013/V42/I1/52


半胱氨酰白三烯受体CysLT1R和CysLT2R以及
GPR17在帕金森病模型小鼠脑内的表达分布

目的:探讨MPTP小鼠帕金森病(PD)模型脑内半胱氨酰白三烯受体CysLT1R、CysLT2R和GPR17表达分布特征。
方法:C57BL/6小鼠连续5 d皮下注射MPTP 25 mg/kg诱导PD模型,第10 d 处死动物。应用免疫组化方法,观察CysLT1R、CysLT2R和GPR17在黑质表达的变化,并用免疫双标方法进行细胞定位。
结果:正常小鼠黑质中有CysLT1R、CysLT2R和GPR17表达,主要表达在TH阳性多巴胺能神经元以及小胶质细胞,CysLT2R还表达于星形胶质细胞;其中,在多巴胺能神经元中91%表达GPR17,77%表达CysLT1R,52%表达CysLT2R。与对照组比较,PD模型组小鼠黑质中TH阳性细胞数显著减少;同时CysLT1R、CysLT2R和GPR17阳性细胞数以及表达CysLT1R、CysLT2R和GPR17的多巴胺细胞数均显著减少。在正常小鼠纹状体中有CysLT1R、GPR17表达,主要表达于神经元;而CysLT2R表达很少,主要表达于星形胶质细胞。PD模型组小鼠纹状体中CysLT1R、GPR17阳性细胞数减少;但CysLT2R表达升高,主要表达于增生的星形胶质细胞。
结论:CysLT1R、CysLT2R和GPR17可能参与MPTP所致的小鼠PD损伤过程。
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