Gas chromatography-mass spectrometry; Oils,volatile/analysis; Aucklandiae Radix/analysis; Lactones/analysis; Staining and labeling; Fluoresceins; Hepatitis,toxic," /> Gas chromatography-mass spectrometry; Oils,volatile/analysis; Aucklandiae Radix/analysis; Lactones/analysis; Staining and labeling; Fluoresceins; Hepatitis,toxic,"/> Gas chromatography-mass spectrometry; Oils,volatile/analysis; Aucklandiae Radix/analysis; Lactones/analysis; Staining and labeling; Fluoresceins; Hepatitis,toxic,"/> 木香肝毒性组分筛查与GC-MS分析研究
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Journal of ZheJiang University(Medical Science)  2012, Vol. 41 Issue (1): 43-46    DOI:
    
Screening and identifying hepatotoxic components in Aucklandiae Radix with GC-MS

ZHAO Xiao-ping 1,2,LU Lin 2,HU Bin3,WANG Shu-fang3
1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China; 2.Zhejiang Chinese Medicine University,Hangzhou 310053,China; 3.College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China
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Abstract  Objective: To screen the potential hepatotoxic components in Chinese herb medicine Aucklandiae Radix.
Methods: The potential hepatotoxic components were screened using HepG2 cells labeled with fluorescein diacetate from 25 fractions of Aucklandiae Radix,in which the hepatotoxic compounds were further identified with GC-MS.
Results: Ten potential hepatotoxic fractions were screened. The identification results by GC-MS indicated that the main compounds in C09 were dehydrocostuslactone,santamarine(or magnolialide) and reynosin,and in C11 were α-costol and elemol.
Conclusion: Dehydrocostuslactone,santamarine(or magnolialide),reynosin,α-costol and elemol are potential hepatotoxic compounds in Aucklandiae Radix.


Key wordsGas chromatography-mass spectrometry; Oils')" href="#">

Gas chromatography-mass spectrometry; Oils      volatile/analysis; Aucklandiae Radix/analysis; Lactones/analysis; Staining and labeling; Fluoresceins; Hepatitis      toxic     

Published: 25 January 2012
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. Screening and identifying hepatotoxic components in Aucklandiae Radix with GC-MS. Journal of ZheJiang University(Medical Science), 2012, 41(1): 43-46.

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http://www.zjujournals.com/med/     OR     http://www.zjujournals.com/med/Y2012/V41/I1/43


木香肝毒性组分筛查与GC-MS分析研究

目的:筛查中药材木香中潜在的肝毒性组分。
方法:对木香25个化学组分,采用二乙酸荧光素荧光标记法在HepG2细胞模型上筛查肝毒性组分,并使用气相色谱-质谱联用仪分析其化学组成。
结果:从木香中筛查出10个肝毒性组分,经气相色谱-质谱联用定性分析,C09组分的主要成分为去氢木香内酯、santamarine(或magnolialide)和reynosin,而C11组分的主要成分是α-木香醇和榄香醇。
结论:木香所含的去氢木香内酯、santamarine(或magnolialide)、reynosin、α-木香醇和榄香醇可能具有肝毒性。

关键词: 气相色谱-质谱法,  油类,  挥发/分析,  木香/分析,  内酯类/分析,  染色与标记,  荧光素类,  肝炎,  中毒性 
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