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Journal of ZheJiang University(Medical Science)  2012, Vol. 41 Issue (1): 32-42    DOI:
    
Identification of major components of traditional Chinese medicine Naodesheng tablet by HPLC-DAD-MSn
CHEN Hui-zhen,LI Yun-fei,ZHONG Jing-hua,FAN Xiao-hui
College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China
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Abstract  Objective: To identify the major components of traditional Chinese medicine Naodesheng tablet.
Methods: A HPLC-DAD-MSn based method was developed to analyze and identify the major components of Naodesheng tablet.Separation was performed on an Agilent Zorbax SB-C18 column(4.6 mm×250 mm,i.d,5 μm) with mobile phase consisting of water with 0.05% formic acid and acetonitrile as gradient eluent at the flow rate of 0.5 ml·min-1.
Results: A total of 43 components were detected,among which 22 were identified by comparing their UV absorption profiles,the information of molecular weights,and structures provided by ESI-MSn with those of available standards and reference data,such as Safflor yellow A,4′-O-Glucosylpuerarin,3′-hydroxypuerarin, Genistein-8-C-apiosy(1-6) glucoside,Puerarin,6″-O-xylosylpuerarin,6″-O-apiosylpuerarin, 3′-methoxypuruerarin,3′-methoxy-6″-o-xylosylpuerarin,Daidzin,Genistin,Pueroside A,Notoginsenoside R1,Ginsenoside Re,Ginsenoside Rg1,Daidzein,Biochanin A,Ginsenoside Rb1,Ginsenoside Rc,Ginsenoside Rb2,Ginsenoside Rb3,Ginsenoside Rd.
Conclusion: The proposed method can identify the main components of Naodesheng tablet and provide information for the quality control of this medicine.


Key wordsPueraria lobata/chemistry      Carthamus tinctorius/chemistry      Ligusticum chuanxiong/chemistry      Panax notoginseng/chemistry      Chromatography, high pressure liquid      Chromatography      Mass spectrometry      Drugs, Chinese herbal     
Published: 25 January 2012
Cite this article:

. Identification of major components of traditional Chinese medicine Naodesheng tablet by HPLC-DAD-MSn. Journal of ZheJiang University(Medical Science), 2012, 41(1): 32-42.

URL:

http://www.zjujournals.com/med/     OR     http://www.zjujournals.com/med/Y2012/V41/I1/32


脑得生片物质基础的HPLC-DAD-MSn分析

目的:明确脑得生片的化学物质基础。
方法:采用高效液相-二极管阵列-质谱(HPLC-DAD-MSn)联用技术检测并鉴定其主要化学成分。色谱柱为Agilent Zorbax SB-C18(4.6 mm×250 mm,i.d,5 μm),流动相为0.05%甲酸-水和乙腈,流速为0.5 ml·min-1。
结果:在脑得生片中共检测到43个化学成分,通过比对紫外吸收图谱、标准品ESI-MSn质谱图提供的分子量及结构式,并结合文献报道,推断鉴定出其中22个化合物,分别为羟基红花黄色素A、4′-O-葡萄糖基葛根素、3′-羟基葛根素、大豆苷元-8-C-芹菜糖基(1-6)葡萄糖苷、葛根素、6″-O-木糖基葛根素、6″-O-芹菜糖基葛根素、3′-甲氧基葛根素、3′-甲氧基-6″-O-木糖葛根素、大豆苷、5,7-三羟基异黄酮-7-糖苷、葛根苷A、三七皂苷 R1、人参皂苷 Re、人参皂苷 Rg1、大豆苷元、鹰嘴豆素 A、人参皂苷 Rb1、人参皂苷Rc、人参皂苷Rb2、人参皂苷Rb3和人参皂苷Rd。
结论:初步明确了脑得生片的化学物质基础,为进一步提高脑得生片的质量控制标准提供了科学数据。

关键词: 葛根/化学,  红花/化学,  川芎/化学,  三七/化学,  色谱法,  高压液相,  色谱法,  质谱分析法 
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