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Food Qual Safet
Food Qual Safet
    
Detection of antibiotic resistance toxigenic Clostridium difficile in processed retail lettuce
Yi Han, Joan King, Marlene E. Janes
School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA
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Abstract  
Objectives: Clostridium difficile is the major cause of infectious diarrhoea in humans after antimicrobial treatment. Clostridium difficile has been isolated from food animals and meat. The main purpose of this study was to characterize C. difficile isolated from retail lettuce and determine the antibiotic resistance using five common clinical-selected antibiotics (metronidazole, vancomycin, clindamycin, erythromycin, and cefotaxime).
Materials and Methods: Lettuce samples (grown in California, Arkansas, and Louisiana) were purchased from retail stores.
Results: Toxigenic C. difficile was isolated from 13.8 per cent (41/297) of the lettuce samples. Among the toxigenic isolates, only 82.9 per cent (34/41) produced toxin B, 17.1 per cent (7/41) produced both toxin A and toxin B, and two of the Louisiana C. difficile isolates were identified as ribotype 027. Under the treatment of the five antibiotics, the virulence C. difficile isolates were identified as having antibiotic resistance to metronidazole, vancomycin, and erythromycin.
Conclusion: The present study reports the highest prevalence of toxigenic C. difficile in US retail lettuce. The antibiotic resistance to metronidazole, vancomycin, and erythromycin of the isolated C. difficile from retail lettuces could lead to public health concerns.


Key words： Clostridium difficile      lettuce      toxigenic      toxin A      toxin B      antibiotic     
Published: 01 March 2018
Corresponding Authors: Marlene E. Janes, School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA     E-mail: Mjanes@agcenter.lsu.edu
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Yi Han
Joan King
Marlene E. Janes
Cite this article:

Yi Han, Joan King, Marlene E. Janes. Detection of antibiotic resistance toxigenic Clostridium difficile in processed retail lettuce. Food Qual Safet, 2018, 2(1): 37-41.

URL:

http://www.zjujournals.com/fqs/10.1093/fqsafe/fyx032     OR     http://www.zjujournals.com/fqs/Y2018/V2/I1/37


零售生菜中抗生素耐药性艰难梭菌的检测

【目标】艰难梭菌是抗菌治疗后人类感染性腹泻的主要原因。已经从食源性动物和肉类中分离出艰难梭状芽孢杆菌。本研究的主要目的是从零售生菜中分离鉴定艰难梭菌，并使用五种临床常用抗生素（甲硝唑、万古霉素、克林霉素、红霉素和头孢菌素）确定抗生素耐药性。

【材料和方法】生菜样品购买自加利福尼亚州、阿肯色州和路易斯安那州的零售店。

【结果】13.8％的莴苣样品中分离出致病性艰难梭菌。 在产毒分离株中，仅有82.9％产生外毒素B，17.1％产生外毒素A和毒素B，并且两种艰难梭菌被鉴定为核糖型027.在五种抗生素作用下，艰难梭菌分离株被鉴定为对甲硝唑，万古霉素和红霉素具有抗生素耐药性。

【结论】本研究报告得出美国零售生菜中产毒性艰难梭菌流行率最高。来自零售生菜分离的艰难梭菌对甲硝唑、万古霉素和红霉素等抗生素耐药性可能导致公共健康问题。


关键词： 艰难梭菌生菜,  产生毒素的,  毒素 A,  毒素 B,  耐药性 
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