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Food Qual Safet
Research Articles     
基于川明参中根和叶脂肪酸图谱对不同产地的鉴别
Discrimination of Chuanminshen violaceum Sheh et Shen from different regions based on fatty acid profiles of roots and leaves
Qing Zhang*, Junrui Tian*, Chong Wan*, Hongmin Dong**, Dingtao Wu*, Shuxiang Liu* and Wen Qin*
*College of Food Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya’an 625014, Sichuan, China; **Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada
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摘要:

目的:建立四川不同地区川明参地理来源的可靠判别方法。

材料与方法:采用气相色谱-质谱联用(GC-MS)和多元统计分析法,包括正交偏最小二乘判别分析和聚类分析,测定了四川省广元、阆中、金堂、巴中、双岭等不同地区川明参的根和叶中脂肪酸图谱。

结果:川明参叶子中十六碳三烯酸(3.21g/kg)、亚麻酸(6.62g/kg)、α-亚麻酸(7.24g/kg)含量最高,均高于根中含量。根据叶片脂肪酸图谱可以清楚地区分从阆中、金堂和广元采集的川明参药材。根据根的脂肪酸图谱可以清楚地区分从阆中、广元和巴中采集的川明参药材。

结论:化学计量学方法可作为鉴别川明参及其炮制品质量的根、叶脂肪酸谱分析方法。

关键词: 川明参脂肪酸谱地理判别正交偏最小二乘判别分析层次聚类分析    
Abstract:

Objectives

The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan, China.

Materials and methods

Fatty acid profiles of roots and leaves of C. violaceum planted in various regions of Sichuan Province in China, namely Guangyuan (GY), Langzhong (LZ), Jintang (JT), Bazhong (BZ), and Shuangling (SL), were determined using GC-MS followed by multivariate statistical analyses, including orthogonal partial least-squares discriminant analysis and hierarchical clustering analysis. 

Results

Leaves of C. violaceum showed the highest contents of hexadecatrienoic acid (3.21 g/kg), linoleic acid (6.62 g/kg), and α-linolenic acid (7.24 g/kg), which were all higher than those contained in roots. Chuanminshen violaceum samples collected from LZ, JT, and GY could be clearly distinguished based on fatty acid profiles of leaves and those collected from LZ, GY, and BZ could be clearly distinguished based on fatty acid profiles of roots.

Conclusions

Chemometric method is used as a potential approach for analyses of fatty acid profiles of roots and leaves to control the quality of C. violaceum and their powered products.

Key words: Chuanminshen violaceum Sheh et Shan    fatty acid profiles    geographical discrimination    orthogonal partial least-squares discriminant analysis    hierarchical clustering analysis
通讯作者: Qing Zhang     E-mail: zhangqing@sicau.edu.cn
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引用本文:

Qing Zhang, Junrui Tian, Chong Wan, Hongmin Dong, Dingtao Wu, Shuxiang Liu, Wen Qin. Discrimination of Chuanminshen violaceum Sheh et Shen from different regions based on fatty acid profiles of roots and leaves. Food Qual Safet, 10.1093/fqsafe/fyaa010.

链接本文:

http://www.zjujournals.com/fqs/CN/10.1093/fqsafe/fyaa010        http://www.zjujournals.com/fqs/CN/Y2020/V4/I2/91

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