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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (9): 675-682    DOI: 10.1631/jzus.B0920077
Biomedicine     
DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters
Qi-lian LIANG, Bi-rong WANG, Guo-hong LI
Department of Medical Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
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Abstract  Objective: To investigate the expression of death decoy receptor 3 (DcR3) and survivin in colorectal carcinoma. Methods: Tumor and normal tissues were taken from a total of 100 colorectal carcinoma patients during surgery, and the expression of DcR3 and survivin was examined by immunohistochemistry, Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) analyses. Results: RT-PCR showed that the expression levels of DcR3 mRNA (0.846±0.242, P<0.01) and survivin mRNA (0.7835±0.2392, P<0.01) in colorectal cancer tissues were significantly higher than those in adjacent normal tissues. Western blotting showed that the expression levels of DcR3 protein (0.795±0.261, P<0.01) and survivin protein (0.6765±0.1351, P<0.01) in tumor tissues were significantly higher than those in non-cancer tissues. The immunohistochemical streptavidin-peroxidase (SP) method showed that the positive expression rates of DcR3 and survivin were 67.0% and 58.0% in colorectal cancer tissues, and 18.0% and 3.0% in non-cancerous colorectal tissues (P<0.05), respectively. The positive correlations of DcR3 (P<0.01) and survivin (P<0.01) to the differentiation of colorectal carcinoma cells, lymph node metastasis, and pathological stage were observed. The expression of DcR3 and survivin was found to be positively correlated to clinicopathologic parameters of colorectal carcinoma. Conclusion: The overexpressed DcR3 and survivin in colorectal cancer may contribute to the development of the cancer. The monitoring of these two proteins may be useful for the diagnosis, differentiation, metastasis, and determination of stages of colorectal carcinoma.

Key words： Death decoy receptor 3 (DcR3)      Survivin      Colorectal carcinoma     
Received: 03 March 2009     
CLC:  R735  
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Qi-lian LIANG
Bi-rong WANG
Guo-hong LI
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Qi-lian LIANG, Bi-rong WANG, Guo-hong LI. DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(9): 675-682.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0920077     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I9/675

[1] XU Dong, LI Xu-fen, ZHENG Shu, JIANG Wen-zhi. Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(6): 445-451.
[2] ZHAO Dan-ping, DING Xiao-wen, PENG Jia-ping, ZHENG Yi-xiong, ZHANG Su-zhan. Prognostic significance of bcl-2 and p53 expression in colorectal carcinoma[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(12): 5-.