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浙江大学学报(工学版)
J4  2010, Vol. 44 Issue (2): 320-325    DOI: 10.3785/j.issn.1008-973X.2010.02.020
化学工程﹑环境工程     
外循环气升式生物反应器中酯酶生产及应用
沈雪亮1, 范永仙2, 汪钊1
（1. 浙江工业大学 生物与环境工程学院,浙江 杭州 310014; 2. 浙江工业大学 化学工程与材料学院,浙江 杭州 310014）
Esterase fermentation in an external-loop airlift bioreactor and its application in D-lactic acid production
SHEN Xue-liang1, FAN Yong-Xian2, WANG Zhao1
(1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China；
2. College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, China)
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摘要：

采用海藻酸钙凝胶包埋法固定根霉（Rhizopus sp. F-16）细胞,在外循环气升式生物反应器（工作体积为10 L,高径比为2.9）中发酵生产酯酶.与游离细胞发酵相比,固定化细胞具有发酵时间短、产酶活力高等优点,其最适通气量为0.70 m3/h,最适装液量为8.75 L,发酵48 h时酯酶活力达41.6 U/g细胞.固定化细胞的适宜作用pH为8.0～9.0,适宜作用温度为45～50 ℃,其酸碱稳定性和热稳定性均比游离细胞明显增强.该固定化细胞可直接用于选择性水解外消旋乳酸乙酯生成D-乳酸,当底物的质量浓度为150 g/L,反应时间为3 h时乳酸乙酯的水解率达43.9%,D-乳酸的光学纯度达95.7%.固定化细胞性状稳定,可重复利用,连续降解6批乳酸乙酯的平均水解率为43.3%,D-乳酸的光学纯度平均为95.4%.
Abstract:

The cells of Rhizopus sp. F-16 were immobilized by being entrapped into calcium alginate gels. The immobilized cells were cultivated in an external-loop airlift bioreactor (working volume 10 L and height-to-diameter ratio 2.9) for the production of esterase. The fermentation term of immobilized cells was shortened and the activity of esterase was improved compared with free cells. The optimum air flow rate and liquid volume for the fermentation of immobilized cells were 0.70 m3/h and 8.75 L, respectively. The esterase activity reached 41.6 U/g cell after 48 h fermentation. The suitable reaction pH value and the temperature of immobilized cells were 8.0～9.0 and 45～50 ℃, respectively. The acidic and alkali stability and the thermal stability of immobilized cells were both enhanced than free cells. The immobilized cells can hydrolyze racemic ethyl lactate enantioselectively to produce D-lactic acid. When the substrate mass concentration was 150 g/L, the hydrolysis yield of ethyl lactate was 43.9% and the optical purity of D-lactic acid reached 95.7% after 3 h reaction. The immobilized cells were quite stable and can be repeatedly used for at least 6 batches of resolution reaction. The average hydrolysis yield of ethyl lactate was 43.3%, and the average optical purity of D-lactic acid was 95.4%.
出版日期: 2010-03-09
:  TQ 925  
通讯作者: 汪钊,男,教授.     E-mail: hzwangzhao@163.com
作者简介: 沈雪亮（1977—）,女,浙江杭州人,副教授,从事生物工程研究.
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引用本文:

沈雪亮, 范永仙, 汪钊. 外循环气升式生物反应器中酯酶生产及应用[J]. J4, 2010, 44(2): 320-325.

CHEN Xue-Liang, FAN Yong-Xian, HONG Zhao. Esterase fermentation in an external-loop airlift bioreactor and its application in D-lactic acid production. J4, 2010, 44(2): 320-325.

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http://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2010.02.020    或    http://www.zjujournals.com/eng/CN/Y2010/V44/I2/320

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