Aseptic germination and bulblet formation of <i>Ixiolirion tataricum</i> (Pall.) Herb. seeds.

doi:10.3785/j.issn.1008-9209.2018.07.041

Journal of Zhejiang University (Agriculture and Life Sciences)

2019, Vol. 45

Issue (3): 296-305 DOI: 10.3785/j.issn.1008-9209.2018.07.041

Horticulture

Aseptic germination and bulblet formation of Ixiolirion tataricum (Pall.) Herb. seeds.

Xuesi Lü(

),Ziming REN,Dong ZHANG,Yiping XIA(

)

Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

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Abstract

Ixiolirion tataricum (Pall.) Herb. is a unique and elite plant germplasm resource in China. The distribution of I. tataricum is limited in Xinjiang Uygur Autonomous Region. Ixiolirion. tataricum has strong resistance, high ornamental and medicinal values, which are of great potential in breeding. In this study, the seeds of I. tataricum collected from Xinjiang Uygur Autonomous Region were used as materials to compare the effects of sterilization method, culture conditions, gibberellin A₃(GA₃) pretreatment and storage conditions on the aseptic germination and bulblet formation. The results showed that 30 s treatment with 75% ethanol followed by 5 min treatment with 2% NaClO was the best sterilization method for I. tataricum with 100% sterile seedlings. Using this sterilization method, the germination rate was more than 94.0% at 4 ℃, while almost no seed germinated at 25 ℃. Normally, seeds could germinate after storage for six months in the refrigerator at 4 ℃, but completely lost germination capacity at room temperature. We also found that MS+6-benzylaminopurine (6-BA) 1.0 mg/L+1-naphthylacetic acid (NAA) 0.2 mg/L+sucrose 30 g/L was the best germination medium for I. tataricum, which was beneficial to bulblet formation. Germinated in this medium, shoots could eventually expanded into bulbs after being transferred to light culture at 25 ℃ and then transferred to MS medium containing 60 g/L sucrose. This study provides an experimental basis for in vitro conservation of I. tataricum and to enhance the development and utilization of wild resources in China.

Key words： Ixiolirion tataricum (Pall.) Herb. aseptic seeding resource preservation seed germination bulblet formation

Received: 04 July 2018 Published: 25 June 2019

CLC:

S 682.2

Corresponding Authors: Yiping XIA E-mail: lvxuesi2016@zju.edu.cn;ypxia@zju.edu.cn

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Cite this article:

Xuesi Lü,Ziming REN,Dong ZHANG,Yiping XIA. Aseptic germination and bulblet formation of Ixiolirion tataricum (Pall.) Herb. seeds.. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(3): 296-305.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2018.07.041 OR http://www.zjujournals.com/agr/Y2019/V45/I3/296

鸢尾蒜种子无菌萌发及鳞茎形成

以采自新疆的野生鸢尾蒜[Ixiolirion tataricum (Pall.) Herb.]种子为材料，比较研究了消毒方式、培养条件、种子预处理、贮藏条件对鸢尾蒜种子无菌萌发及离体鳞茎形成的影响。无菌播种结果表明：75%乙醇涡旋30 s，结合2% NaClO 5 min是种子的最佳消毒方式，污染率为0；鸢尾蒜种子在4 ℃条件下的萌发率在94.0%以上，而在25 ℃条件下则几乎不萌发。贮藏条件对种子萌发影响很大，4 ℃贮藏6个月后种子依旧能够正常萌发，而室温（25 ℃）贮藏6个月后种子完全丧失发芽力。筛选出的最有利于鸢尾蒜离体鳞茎形成的种子萌发培养基为MS+6-苄基腺嘌呤（6-benzylaminopurine, 6-BA）1.0 mg/L+萘乙酸（1-naphthylacetic acid, NAA）0.2 mg/L+蔗糖30 g/L，在该培养基上播种30 d后出芽整齐，然后转入25 ℃光照条件下，再转接至含60 g/L蔗糖的MS培养基上，可形成膨大的鳞茎。总之，该研究建立的鸢尾蒜无菌播种和鳞茎形成体系，为野生鸢尾蒜的离体资源保存与进一步开发利用提供了理论依据。

关键词： 鸢尾蒜, 无菌播种, 资源保存, 种子萌发, 鳞茎形成

Table 1 Sterilization methods

Table 2 Eleven different treatment conditions for the seeds

Table 3 Seed germination under different sterilization methods

Fig.1 Effects of sterilization methods on seed germination

Fig.2 Bacterial contamination of seeds sterilized by HgCl₂ in seedling stage

Table 4 Seed germination under different culture conditions

Fig.3 Effects of culture conditions on seed germination

Table 5 Seed germination under GA₃ or water soaking treatment

Table 6 Seed germination under different storage conditions

Fig. 4 Plant state changes after transferring to 25 ℃ in daylight

Fig. 5 Plant state after transfer to 25 ℃ in daylight for 70 d

Fig. 6 Morphological indexes of plants in different mediums after 100 d

Fig. 7 Bulblet status after transferring plants to MS medium for 30 d


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