| Resource utilization & environmental protection |
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| Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method |
Longzao LUO1,2( ),Fanjian ZENG2,Guangming TIAN2() |
1. School of Chemistry and Environmental Science, Shangrao Normal University, Shangrao 334001, Jiangxi, China
2. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China |
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Abstract An algal-bacterial system was constructed to explore the effects of culture conditions on the growth of microalgae. On the basis of the results of single factor test, three factors including the agitating rate, the initial inoculation ratio of microalgae and bacteria, and the organic load of the wastewater were optimizated by using the response surface method. The results showed that the microalgal growth was significantly affected by airtight condition, agitation rate, initial inoculation ratio and organic load of the culture system, and the microalgae grew better in the open culture system. The optimum condition by the response surface optimization was as follows: agitating rate of 1 574.29 r/min, inoculation ratio 150∶1 of bacteria-alga and organic load (chemical oxygen demand, COD) of 3 676.02 mg/L. After seven days of culture under the optimimum condition, the biomass of microalgae was 5.68 g/L, which was in accordance with the theoretically predicted value (5.69 g/L). The above results can lay a scientific basis for improving the resource recovery efficiency from wastewater by algal-bacterial system.
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Received: 09 February 2018
Published: 28 March 2019
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Corresponding Authors:
Guangming TIAN
E-mail: luolongzao@sina.com;gmtian@zju.edu.cn
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藻-菌系统中微藻生长条件的响应面法优化
人工构建藻-菌系统，探讨培养系统密闭情况及搅拌速率、藻-菌初始接种比例和废水有机负荷对系统中微藻生长的影响。在单因素试验结果的基础上，利用响应面法对搅拌速率、藻-菌初始接种比例和废水有机负荷3个因素进行优化。结果表明，培养系统密闭情况及搅拌速率、藻-菌初始接种比例和废水有机负荷均显著影响系统中微藻的生长，其中培养系统敞开更有利于微藻的生长。经响应面优化的最佳培养条件为：搅拌速率1 574.29 r/min，菌-藻接种比例150∶1，有机负荷（以化学需氧量计）3 676.02 mg/L。在此条件下培养7 d后，微藻的生物量为5.68 g/L，与理论预测值（5.69 g/L）基本吻合。本研究结果可为提高藻-菌系统对废水的资源化利用效率提供科学依据。
关键词：
微藻,
藻-菌系统,
培养条件,
响应面法,
优化
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