Please wait a minute...
浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2018, Vol. 44 Issue (6): 722-726    DOI: 10.3785/j.issn.1008-9209.2017.12.132
    
c-Jun protein effect on regulating CD4＋ and CD8＋ T cell proliferation in H1N1 influenza A virus infected mice
ZHANG Shouping1,2, SHEN Wentao1, WANG Lirong1, ZHANG Baizhong3*
(1. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; 2. College of Veterinary Medicine, China Agricultural University, Beijing 100192, China; 3. College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China)
Download: HTML ( )    PDF(1826KB)
Export: BibTeX | EndNote (RIS)      

Abstract  A H1N1 influenza A virus infected mice model was built to evaluate the regulation mechanism of c-Jun protein in T lymphocytes. To determine the DNAzyme Dz13 efficiency in virus-infected animals, a Westernblotting assay was employed to detected total c-Jun (t-c-Jun) and phosphorylation c-Jun (p-c-Jun) levels by specific antibody. It was demonstrated that the drug-treated mice markedly suppressed the expression of t-c-Jun and p-c-Jun. Through flow cytometry and quantitative real-time polymerase chain reaction (qRT-PCR), we could find an obvious CD4＋ (helper T lymphocytes) and CD8＋ T (cytotoxic T lymphocyte) cell proliferation and relative cytokine (IFN-γ, IL-6 and IL-10) expression on day 3 and 6 in the virus-infected mice. The Dz13-treated mice with a c-Jun suppression displayed a down-regulation of CD4＋ and CD8＋ T cells in peripheral blood. At the same time, Dz13-treated group demonstrated a significant reduction of inflammatory cytokine (IFN-γ, IL-6 and IL-10) expression. In conclusion, c-Jun protein regulates CD4＋ and CD8＋ T cell proliferation and inflammatory cytokine expression in H1N1 influenza A virus infected mice.

Key words： c-Jun protein      influenza A virus      T lymphocyte      cytokine     
Published: 25 November 2018
CLC:  Q 28  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
ZHANG Shouping
SHEN Wentao
WANG Lirong
ZHANG Baizhong
Cite this article:

ZHANG Shouping, SHEN Wentao, WANG Lirong, ZHANG Baizhong. c-Jun protein effect on regulating CD4＋ and CD8＋ T cell proliferation in H1N1 influenza A virus infected mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(6): 722-726.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2017.12.132     OR     http://www.zjujournals.com/agr/Y2018/V44/I6/722


c-Jun 蛋白对A 型流感病毒H1N1 感染小鼠体内CD4＋和 CD8＋ T 细胞增殖的调节作用

通过构建Ａ型流感病毒H1N1 感染小鼠模型，并使用c-Jun 蛋白的特异性核酶Dz13 抑制该蛋白的表达，探索c-Jun 蛋白在流感病毒感染后对T淋巴细胞增殖和炎性反应的调节作用。蛋白质印迹法（Western-blotting）结果表明，核酶Dz13 作用后c-Jun 蛋白的表达量明显降低，且c-Jun 蛋白的活化也随之降低。此外，用流式细胞术测定感染后3 d 和6 d 外周血内辅助性T细胞（CD4＋T）和杀伤性T细胞（CD8＋T）的增殖情况，同时用实时荧光定量聚合酶链式反应测定外周血内细胞因子IFN-γ、IL-6 和IL-10 表达。结果显示：小鼠感染病毒后，外周血CD4＋和CD8＋T细胞明显增多，相关细胞因子的表达量也明显上调；而抑制c-Jun 蛋白表达后，CD4＋和CD8＋ T细胞的增殖受到抑制，且相关细胞因子IFN-γ、IL-6 和IL-10 的表达量亦受到抑制。说明c-Jun 蛋白参与调节A型流感病毒H1N1 感染后引起的T淋巴细胞增殖和相关细胞因子的表达。


关键词： c-Jun 蛋白,  A型流感病毒,  T淋巴细胞,  细胞因子 
[1] Chen Shanshan, Luo Qihui, Zeng Wen, Cheng Anchun, Liu Wentao, Shi Liangqin, Chen Zhengli. Expression of Th1/Th2 cytokines in partial target organs of type 2 diabetes mellitus rhesus monkey[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(03): 302-308.