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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2018, Vol. 44 Issue (6): 727-734    DOI: 10.3785/j.issn.1008-9209.2017.08.212
    
Effects of 5- azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells
YU Mengfei1,2?, WANG Wenlu2?, YI Jianming2*
(1. Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China/Institute for Medical Biology, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China; 2. Key Laboratory of Agricultural Animal Genetic, Breeding, and Reproduction of Ministry of Education, College of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China)
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Abstract  Fetal bovine fibroblast cells (FBFCs) are widely used in cellular reprogramming and methylation. In this study, the effects of 5- azacytidine-2’- deoxycytidines (5-Aza-CdR), a DNA methylation inhibitor on the physiological characteristics of FBFCs were investigated. The FBFCs derived from a bovine fetus were treated with 5-Aza-CdR at different concentrations for 24, 48 and 72 h, respectively, to determine the changes of cell proliferation, viability, cell cycle distribution and apoptosis rates. The results demonstrated that 5- Aza- CdR treatment in the FBFCs resulted in a decrease of cell proliferation, cell viability, and an increase of total cell apoptosis in a dose-dependent manner. The best treatment strategy for 5-Aza-CdR was less than 0.1 μmol/L for 24 h, which showed minimal adverse effects on FBFCs. The above results indicate that the low dose DNA demethylation inhibitor treatment in FBFCs is beneficial for cell reprogramming. 

Key words： 5-azacytidine-2’-deoxycytidines      cell proliferation      cell cycle      cell apoptosis     
Published: 25 November 2018
CLC:  Q 253  
  Q 28  
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YU Mengfei
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YU Mengfei, WANG Wenlu, YI Jianming. Effects of 5- azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(6): 727-734.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2017.08.212     OR     http://www.zjujournals.com/agr/Y2018/V44/I6/727


5-氮杂-2’-脱氧胞苷对胎牛成纤维细胞增殖、周期和凋亡的影响(英文)

胎牛成纤维细胞是细胞重编程和去甲基化研究的常用材料。本文以胎牛成纤维细胞为材料，用不同浓度的DNA去甲基抑制剂药物5-氮杂-2’-脱氧胞苷分别处理24、48 和72 h 后，研究其对胎牛成纤维细胞的增殖、活力、细胞周期分布和凋亡率的影响。结果显示，5-氮杂-2’-脱氧胞苷以浓度依赖性的方式抑制胎牛成纤维细胞的增殖、活力，并增加细胞的总凋亡率。其中，用浓度低于0.1 μmol/L 的5-氮杂-2’-脱氧胞苷处理24 h 时，胎牛成纤维细胞受到的不利影响最小。这也说明采用相对低浓度的DNA去甲基抑制剂处理体细胞对于细胞的重编程是有益的。

关键词： 5-氮杂-2’-脱氧胞苷,  细胞增殖,  细胞周期,  细胞凋亡 
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