Heat tolerance evaluation of transgenic cotton germplasms with insect resistance and herbicide tolerance

doi:10.3785/j.issn.1008-9209.2018.12.051

浙江大学学报（农业与生命科学版）

2019, Vol. 45

Issue (6): 647-656 DOI: 10.3785/j.issn.1008-9209.2018.12.051

作物栽培与生理

Heat tolerance evaluation of transgenic cotton germplasms with insect resistance and herbicide tolerance

Elmon CHINDUDZI(

),苏帮荣,郭伊,钟镇涛,Jane MAKONI,祝水金,陈进红(

)

浙江大学农业与生物技术学院，杭州 310058
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Elmon CHINDUDZI(

),Bangrong SU,Yi GUO,Zhentao ZHONG,Jane MAKONI,Shuijin ZHU,Jinhong CHEN(

)

全文: PDF(632 KB) HTML

摘要：

在前期利用田间植株进行膜热稳定性、花粉生活力和叶绿素稳定性等耐热评价筛选基础上，选择HNZ 1063、HNZ 1068、HNZ 1073、HNZ 1081、HNZ 1088和HNZ 1091等6个转基因抗虫、耐除草剂棉花种质和常规栽培种中棉所49（ZMS 49），通过室内培养对4周苗龄的幼苗在42 ℃(光照)/24 ℃（黑暗）条件下处理1周，测定其光合速率、叶绿素含量、抗氧化酶活性等指标，以进一步评价参试种质的耐热性。结果表明，HNZ 1091、HNZ 1068和ZMS 49种质较为耐热，而HNZ 1081和HNZ 1073种质耐热性较差。说明插入相同基因的不同的转基因株系的耐热反应存在差异。

关键词： 转基因抗虫耐除草剂棉花种质; 热胁迫; 光合速率; 叶绿素含量; 抗氧化酶活性

Abstract:

Heat stress has increasingly become a global problem affecting agriculture including cotton production which is a critical fiber and oil crop. The purpose of this study was to evaluate six transgenic insect-resistant and herbicide-tolerant cotton germplasms (HNZ 1063, HNZ 1068, HNZ 1073, HNZ 1081, HNZ 1088 and HNZ 1091) and the conventional cultivar Zhongmiansuo 49 (ZMS 49) based on the evaluation and screening of membrane thermostability, pollen viability and chlorophyll stability of field plants as an initial study. A follow up study was conducted using four-week-old seedlings treated at 42 ℃ (light)/24 ℃ (dark) for one week to further evaluate heat tolerance by measuring and analyzing photosynthetic rate, chlorophyll content, antioxidant enzyme activity and growth parameters. The results showed that three germplasms, HNZ 1091, HNZ 1068 and ZMS 49, were more heat-tolerant, while two, HNZ 1081 and 1073, were less tolerant. It can thus be concluded that there are differences in heat tolerance among different transgenic lines inserting the same gene.

Key words: transgenic insect-resistant and herbicide-tolerant cotton germplasms heat stress photosynthetic rate chlorophyll content antioxidant enzyme activity

出版日期: 2020-01-20

CLC:

S 562

基金资助: the National Key Research and Development Project(2018YFD0100401)

通讯作者: 陈进红 E-mail: elmonchindudzi@yahoo.com;jinhongchen@zju.edu.cn

作者简介: CHINDUDZI Elmon (https://orcid.org/0000-0003-0207-5025), E-mail: elmonchindudzi@yahoo.com

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引用本文:

Elmon CHINDUDZI,苏帮荣,郭伊,钟镇涛,Jane MAKONI,祝水金,陈进红. Heat tolerance evaluation of transgenic cotton germplasms with insect resistance and herbicide tolerance[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(6): 647-656.

Elmon CHINDUDZI,Bangrong SU,Yi GUO,Zhentao ZHONG,Jane MAKONI,Shuijin ZHU,Jinhong CHEN. . Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(6): 647-656.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.12.051 或 http://www.zjujournals.com/agr/CN/Y2019/V45/I6/647

Table 1 Heat tolerance selection tests performed at the flowering stage （%）

Fig. 1 Photosynthetic parameters in seven cotton genotypes after exposure to heat stress for one week relative to the control Error bars represent the standard deviation of the mean. Single asterisk (*) shows significant differences between the control and the treatment at the 0.05 probability level.

Fig. 2 Chlorophyll parameters in seven cotton genotypes after exposure to heat stress for one week relative to the control Error bars represent the standard deviation of the mean. Single asterisk (*) shows significant differences between the control and the treatment at the 0.05 probability level.

Fig. 3 Antioxidant and carotenoid parameters in seven cotton genotypes after exposure to heat stress for one week relative to the control Error bars represent the standard deviation of the mean. Single asterisk (*) shows significant differences between the control and the treatment at the 0.05 probability level.

Fig. 4 Growth parameters in seven cotton genotypes after exposure to heat stress for one week relative to the control Error bars represent the standard deviation of the mean. Single asterisk (*) shows significant differences between the control and the treatment at the 0.05 probability level.

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