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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (2): 164-174    DOI: 10.3785/j.issn.1008-9209.2018.06.011
园艺学     
不同种源砂生槐幼苗的生长及光合特征
玉文 赵1(),段少荣1,郑雨2,南吉斌1,米桑1,赵亚磊1,林玲1()
1. 西藏农牧学院资源与环境学院，西藏 林芝 860000
2. 西藏农牧学院高原生态研究所，西藏 林芝 860000
Seedling growth and photosynthetic characteristics of different
Shaorong DUAN1,Yu ZHENG2,Jibin NAN1,Sang MI1,Yalei ZHAO1,Ling LIN1()
1. College of Resources and Environment, Xizang Agriculture and Animal Husbandry College, Linzhi 860000, Xizang, China
2. Research Institute of Tibet Plateau Ecology, Xizang Agriculture and Animal Husbandry College, Linzhi 860000, Xizang, China
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摘要：

为研究砂生槐（Sophora moorcroftiana）种源间生长与光合特性差异，以8个种源砂生槐一年生实生苗为试验材料，测定其生长性状、叶绿素含量、光响应和CO2响应曲线。结果表明：砂生槐幼苗种源间生长性状及叶绿素含量均存在显著差异（P＜0.05）；砂生槐光响应曲线总体呈现为，在一定范围内增加光合有效辐射（photo- synthetically active radiation, PAR），净光合速率（net photosynthetic rate, P n）逐渐增大，达到峰值后，PAR继续增加而各种源P n下降，呈单峰曲线，有明显光抑制现象；种源间光饱和点（light saturation point, L SP）在740.467～1 773.078 μmol/(m2·s)之间，光补偿点（light compensation point, L CP）在1.408～43.381 μmol/(m2·s)之间；林周县和巴宜区种源P n和L SP高于其他种源；CO2响应曲线呈现为，在一定范围内增加CO2浓度，种源间CO2曲线特征参数也存在差异（P＜0.05），CO2饱和点（CO2 saturation point, C SP）在1 769.632～2 678.136 μmol/(m2·s)之间，CO2补偿点（CO2 compensation point, Г）在76.155～111.430 μmol/(m2·s)之间。相关性分析发现：P n与气孔导度（stomatal conductance, G s）和L SP呈极显著正相关（P＜0.01），胞间CO2浓度（intercellular CO2 concentration, C i）与蒸腾速率（transpiration rate, T r）、L CP、暗呼吸速率（dark respiration rate, R d）呈极显著正相关（P＜0.01）。苗高与P n和叶绿素含量呈正相关（P＜0.05）。总之，林周县和巴宜区种源P n较高，其L SP也最高，说明在强光下这2个种源有较高的光合生产潜力。
关键词： 砂生槐;  种源;  幼苗生长;  光照强度;  光合特征    
Abstract:

In order to study the difference on seedling growth and photosynthetic characteristics among Sophora moorcroftiana provenances, the growth characters, chlorophyll content, and curves of light response and CO2 response were analyzed, using eight provenances of one-year-old seedlings. The results showed that there was a significant difference on the growth and chlorophyll content among the provenances of S. moorcroftiana seedlings (P＜0.05). The light response curve showed that the net photosynthetic rate (P n) increased as the increasing photosynthetically active radiation (PAR). When the P n of eight provenances increased to the top, it decreased irrespective of increasing PAR and represented the single-peak curve, with an obvious photo inhibition phenomenon. The light saturation point (L SP) of eight provenances ranged from 740.467 to 1 773.078 μmol/(m2·s), and light compensation point (L CP) ranged from 1.408 to 43.381 μmol/(m2·s). The P n and L SP of provenances in Linzhou County and Bayi District were higher than these of other provenances. The CO2 response curve showed that the concentration of CO2 increased within a certain range. The characteristic parameters of CO2 curve showed statistical differences among the provenances (P＜0.05). The CO2 saturation point (C SP) was between 1 769.632 and 2 678.136 μmol/(m2·s) and the CO2 compensation point (Г) was between 76.155 and 111.430 μmol/(m2·s). Correlation analysis showed that the P n was positively correlated with stomatal conductance (G s) and L SP (P＜0.01), and the intercellular CO2 concentration (C i) was significantly correlated with transpiration rate (T r), L CP and dark respiration rate (R d) (P＜0.01). Seedling height was significantly correlated with P n and the content of chlorophyll (P＜0.05). The provenances of Linzhou County and Bayi District had higher P n, and their L SP were also the highest, which indicated that these two provenances had high photosynthetic potential under the strong light environment.
Key words: Sophora moorcroftiana    provenance    seedling growth    light intensity    photosynthetic characteristics
收稿日期: 2018-06-01 出版日期: 2019-04-25
CLC:  S 792.17  
基金资助: 国家自然科学基金(31560142);西藏自治区自然科学基金(2016ZR-15-38，2016ZX-NY-10);西藏农牧学院研究生创新项目(YJS2018-11)
通讯作者: 林玲     E-mail: 1577125634@qq.com;xizyz@126.com
作者简介: 赵玉文（https://orcid.org/0000-0003-4831-3826），E-mail： 1577125634@qq.com|林玲（https://orcid.org/0000-0002-1786-306X），E-mail： xizyz@126.com
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引用本文:

玉文 赵,段少荣,郑雨,南吉斌,米桑,赵亚磊,林玲. 不同种源砂生槐幼苗的生长及光合特征[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(2): 164-174.

Shaorong DUAN,Yu ZHENG,Jibin NAN,Sang MI,Yalei ZHAO,Ling LIN. Seedling growth and photosynthetic characteristics of different. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(2): 164-174.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.06.011    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I2/164

种源编号

Number

种源区

Provenance

area

纬度

Latitude

N

经度

Longitude

E

海拔

Altitude/m

立地类型

Site type

气候类型

Climate type
BYQ

巴宜区

Bayi District

 29°14.834′ 94°31.180′ 3 014

河滩地砂质土

River beach sandy soil

温带湿润季风气候

Temperate humid monsoon climate
LX

朗县

Lang County

 29°10.374′ 93°32.487′ 3 019

阶地宜林灌草地

Terrace suitable for forest

and grassland

高原温带半干旱季风气候

Semiarid monsoon climate in temperate

zone of the plateau
QSX

曲水县

Qushui County

 29°22.450′ 90°46.651′ 3 597

阶地砂壤土

Terrace sandy soil

高原温带半干旱季风气候

Semiarid monsoon climate in temperate

zone of the plateau
LZX

林周县

Linzhou County

 29°55.095′ 91°16.012′ 3 789

石砾坡地砂土

Gravel slope sandy soil

高原干旱季风气候

Plateau dry monsoon climate
BL

白朗县

Bailang County

 29°09.416′ 89°01.488′ 3 867

高山宽河谷灌草地

Alpine wide valley

irrigated grassland

高原温带半干旱季风气候

Semiarid monsoon climate in temperate

zone of the plateau
XTM

谢通门县

Xietongmen County

 29°21.544′ 88°43.900′ 3 879

阴坡草原棕壤土

Shady steppe brown soil

高原温带半干旱季风气候

Semiarid monsoon climate in temperate

zone of the plateau
AR

昂仁县

Angren County

 29°03.242′ 87°32.950′ 4 037

高寒荒漠砂壤土

Alpine desert sandy soil

温暖少风半干旱气候

Warm, windy and semiarid climate
LZ

拉孜县

Lhaze County

 29°03.212′ 87°54.038′ 4 114

冲积平原草甸土

Alluvial plain meadow soil

高原半干旱季风气候

Semiarid monsoon climate on the plateau
表1  不同种源砂生槐自然地理基本概况

参数

Parameter

 种源 Provenances
BYQ LX QSX LZX BL XTM AR LZ

苗高

Height of seedling/cm

 22.78±1.48c 31.78±0.68a 26.87±1.66b 22.75±0.65c 22.23±0.96c 19.75±0.20d 20.32±0.61d 19.55±0.05d

地径

Ground diameter/mm

 1.99±0.76b 2.84±0.04a 2.11±0.40b 1.86±0.30bc 1.73±0.04c 1.39±0.10e 1.53±0.04d 1.58±0.17d
表2  不同种源砂生槐生长性状比较结果
图1  不同种源砂生槐叶片叶绿素含量
图2  不同种源砂生槐净光合速率、气孔导度、胞间CO2 浓度、蒸腾速率的光响应曲线

种源

Provenance

P n max/

(μmol/(m2·s))

L SP/

(μmol/(m2·s))

L CP/

(μmol/(m2·s))

α/

(μmol/mol)

R d/

(μmol/(m2·s))
BYQ 10.734a 1 348.481b 15.806c 0.050a 0.768b
LX 4.524e 980.922cd 12.313dc 0.035c 0.399d
QSX 4.893e 740.467d 11.949d 0.055a 0.605bc
LZX 11.679a 1 773.078a 8.265d 0.055a 0.445d
BL 7.484b 1 300.953b 1.408e 0.037c 0.051c
XTM 6.340c 788.566d 7.720d 0.056a 0.413d
AR 4.665e 1 218.735b 43.381a 0.043b 1.504a
LZ 5.764d 1 038.533c 29.779b 0.015d 0.434d
表3  不同种源砂生槐光响应曲线的特征参数
图3  不同种源砂生槐净光合速率、气孔导度、胞间CO2 浓度、蒸腾速率的CO2 响应曲线

种源

Provenance

C SP/

(μmol/

(m2·s))

Г/

(μmol/

(m2·s))

V c/

(μmol/mol)

R p/

(μmol/(m2·s))
BYQ 1 769.632e 110.274a 0.026c 2.712c
LX 2 406.369b 76.155c 0.033b 2.371d
QSX 2 310.265c 109.910a 0.026c 2.644c
LZX 2 354.765bc 76.455c 0.051a 3.577a
BL 2 155.230d 91.743b 0.018d 1.545e
XTM 1 881.581d 110.886a 0.031b 3.292b
AR 2 678.136a 94.378b 0.031b 2.833c
LZ 1 964.226d 111.430a 0.032b 3.357ab
表4  不同种源砂生槐CO2响应曲线的特征参数

指标

Index

苗高

Height of seedling

地径

Ground diameter

叶绿素a

Chlorophyll a

叶绿素b

Chlorophyll b

 P n T r C i G s L sp L cp R d
苗高Height of seedling 1
地径Ground diameter 0.973** 1

叶绿素a

Chlorophyll a

 0.599 0.548 1

叶绿素b

Chlorophyll b

 0.755* 0.700 0.923** 1
P n 0.669 0.686 －0.142 －0.408 1
T r －0.334 －0.159 －0.380 －0.348 0.381 1
C i －0.466 －0.379 －0.576 －0.446 －0.116 0.758* 1
G s －0.118 －0.037 0.129 －0.059 0.848** 0.524 －0.055 1
L sp 0.229 0.434 －0.125 －0.344 0.798* 0.489 0.198 0.600 1
L cp －0.333 －0.290 －0.645 －0.471 －0.383 0.409 0.868** －0.474 －0.045 1
R d －0.219 －0.231 －0.452 －0.323 －0.200 0.414 0.792* －0.161 0.034 0.833* 1
表5  砂生槐生长性状与光合特征参数相关性分析
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