超声辅助热水提取对铁皮石斛水溶性多糖得率和结构的影响

doi:10.3785/j.issn.1008-9209.2018.04.161

浙江大学学报（农业与生命科学版）

2019, Vol. 45

Issue (2): 196-204 DOI: 10.3785/j.issn.1008-9209.2018.04.161

食品科学

超声辅助热水提取对铁皮石斛水溶性多糖得率和结构的影响

金蓉^1,²(

),谢文华¹,陈杰标¹,王念晨¹,项白雪¹,王岳¹,曹锦萍¹(

)

1. 浙江大学农业农村部园艺植物生长发育与品质调控重点开放实验室，杭州 310058
2. 浙江大学农业试验站，杭州 310058

Effects of ultrasonic-assisted hot water extraction on the yield and structure of water soluble polysaccharides from Dendrobium officinale

Rong JIN^1,²(

),Wenhua XIE¹,Jiebiao CHEN¹,Nianchen WANG¹,Baixue XIANG¹,Yue WANG¹,Jinping CAO¹(

)

1. Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement of the Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China
2. Agricultural Experimental Station, Zhejiang University, Hangzhou 310058, China

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摘要：

为建立铁皮石斛水溶性多糖超声辅助热水提取法，本文比较了常温和热水提取体系中超声辅助提取对‘圣兰8号’铁皮石斛（Dendrobium officinale）水溶性多糖得率及其分子结构的影响。结果表明：超声辅助提取显著提高了铁皮石斛水溶性多糖得率，并使提取时间从2 h缩短至3 min；超声辅助-热水提取（95～100 ℃）可进一步提高得率，其中500 W超声3 min的效果最佳。凝胶渗透色谱结果显示，超声辅助提取的多糖多分散度显著增高。‘圣兰8号’铁皮石斛水溶性多糖由甘露糖、葡萄糖、半乳糖、阿拉伯糖、鼠李糖、木糖、葡萄糖醛酸和半乳糖醛酸组成，提取方法对多糖红外光谱和单糖组成无明显影响。铁皮石斛水溶性多糖具有刚果红结合能力，超声辅助提取不改变其与刚果红的结合特性。将500 W超声辅助-热水提取方法应用于13个铁皮石斛品种水溶性多糖的提取，发现茎中水溶性多糖质量分数在69.40～130.10 g/kg之间（按鲜质量计），叶片中则在15.30～34.10 g/kg之间（按鲜质量计），品种间存在较大差异。

关键词： 铁皮石斛; 品种; 多糖; 超声辅助提取

Abstract:

Dendrobium officinale is an edible medicinal plant with polysaccharides as its important bioactive components. This study investigated the effects of ultrasonic-assisted extraction on the yield of D. officinale ‘Shenglan No. 8’ water soluble polysaccharides as well as the influence to the molecular structure. The results showed that ultrasonic-assisted methods significantly increased the yield of polysaccharides and shortened the extraction time from 2 h to 3 min. The highest extraction efficiency was obtained in the ultrasonic-assisted hot water synergetic extraction (500 W, 95-100 ℃, 3 min). The results of gel permeation chromatography (GPC) showed that the D. officinale polysaccharides through ultrasonic-assisted extraction were with higher polydispersity (PDI). The water soluble polysaccharides from D. officinale ‘Shenglan No. 8’ were composed of mannose, glucose, galactose, arabinose, rhamnose, xylose, glucuronic acid and galacturonic acid. No significant difference was observed on the infrared spectrum and monosaccharide composition among the different extraction methods. The D. officinale polysaccharides had certain congo red-binding ability, which was not affected by the ultrasonic treatments. The optimum extract parameters (500 W, 95-100 ℃, 3 min) was applied in the comparison of polysaccharide content among 13 cultivars. The polysaccharide content varied among the cultivars and tissues, with a range of 69.40-130.10 g/kg fresh mass in the stem, and a range of 15.30-34.10 g/kg fresh mass in the leaf.

Key words: Dendrobium officinale cultivars polysaccharides ultrasonic-assisted extraction

收稿日期: 2018-04-16 出版日期: 2019-04-25

CLC:

O 629.12

基金资助: 国家重点研发计划(2017YFD0400204-02);高等学校学科创新引智计划（“111”计划）(B17039)

通讯作者: 曹锦萍 E-mail: rong@zju.edu.cn;0017165@zju.edu.cn

作者简介: 金蓉（https://orcid.org/0000-0002-3703-3784），E-mail： rong@zju.edu.cn|曹锦萍（https://orcid.org/0000-0003-0840-5590），E-mail： 0017165@zju.edu.cn

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引用本文:

金蓉,谢文华,陈杰标,王念晨,项白雪,王岳,曹锦萍. 超声辅助热水提取对铁皮石斛水溶性多糖得率和结构的影响[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(2): 196-204.

Rong JIN,Wenhua XIE,Jiebiao CHEN,Nianchen WANG,Baixue XIANG,Yue WANG,Jinping CAO. Effects of ultrasonic-assisted hot water extraction on the yield and structure of water soluble polysaccharides from Dendrobium officinale. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(2): 196-204.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.04.161 或 http://www.zjujournals.com/agr/CN/Y2019/V45/I2/196

表1 在不同提取参数下‘圣兰8号’铁皮石斛水溶性多糖的得率和纯度

图1 不同提取参数下‘圣兰8号’铁皮石斛多糖的GPC图谱

表2 不同提取参数下‘圣兰8号’铁皮石斛水溶性多糖摩尔质量

图2 不同提取参数下‘圣兰8号’铁皮石斛水溶性多糖的刚果红结合能力

图3 不同提取参数下‘圣兰8号’铁皮石斛水溶性多糖的红外图谱

图4 铁皮石斛‘圣兰8号’水溶性多糖中单糖组成的HPLC图谱

表3 不同提取参数下铁皮石斛‘圣兰8号’多糖的单糖组成

图5 不同品种二年生铁皮石斛茎（A）和叶片（B）的多糖含量

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