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浙江大学学报(农业与生命科学版)
论文     
浙江2个地产白沙枇杷种质资源亲缘关系鉴定
陈方永1*, 王引1, 倪海枝1, 张启2, 颜帮国1
(1.浙江省柑橘研究所,浙江 黄岩 318026;2.浙江省兰溪市农业局经济特产技术推广站,浙江 兰溪 321100)
Genetic relationship identification on germplasm resources of two whiteflesh loquats from two different producing areas of Zhejiang Province
CHEN Fangyong1*, WANG Yin1, NI Haizhi1, ZHANG Qi2, YAN Bangguo1
(1. Citrus Research Institute of Zhejiang, Huangyan 318026, Zhejiang, China; 2. Economic Specialty Technology Extension Station, Agricultural Bureau of Lanxi, Lanxi 321100, Zhejiang, China)
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摘要: 在综合观察浙江2个白沙枇杷(黄岩软条白沙和兰溪白沙)种质形态特征的基础上,对两者进行倍性、气孔检测和基于分子标记的亲缘关系研究。结果表明:黄岩软条白沙单果质量是兰溪白沙的1.38倍,而兰溪白沙的种子数、果皮厚度、果实硬度分别是黄岩软条白沙的1.36倍、1.096倍、1.22倍;流式细胞仪倍性检测表明,两者均为二倍体;气孔检测表明,两者的气孔密度和面积大小差异显著,黄岩软条白沙气孔面积比兰溪白沙大43.4%,气孔密度比兰溪白沙小17.35%;基于简单序列重复标记的遗传聚类分析表明,黄岩软条白沙的遗传相似系数为0.40,兰溪白沙为0.45。由此可见,2个品种在成熟期的差异主要受种性和地域因素的影响,可认为是不同的栽培种质。
Abstract: Loquat, Eriobotrya japonica (Thunb.) Lindl., is indigenous in southeast of China, which belongs to the family Rosaceae and subfamily Maloideae. Based on the color of fruit flesh, loquat can be sorted into red- and white-flesh cultivars. Zhejiang Province is the major producing area of loquat in China, with an area of about 1.2×104 hm2; the annual production of loquat is about 6.5×104 t, with a value of about 2.5 billion Chinese Yuan. Therefore, germplasm resources of loquat are abundant in Zhejiang Province. However, there are two white-flesh loquat  germplasms (Ruantiao white-flesh loquat from Huangyan, white-flesh loquat from Lanxi) which are different in leaf morphology and fruit shape from two producing areas of Zhejiang Province. Controversy on whether the two varieties are synonyms has existed for a long time, and no conclusive evidence was presented yet. 
In this paper, morphological characteristics of the two varieties were examined, and their ploidy and stomata were analyzed, and  their genetic relationships were identified using simple sequence repeat (SSR) marker. 
Results showed that the single fruit mass of Ruantiao white-flesh loquat from Huangyan area was 1.38 times heavier than that of whiteflesh loquat from Lanxi area. However, the number of seeds, pericarp thickness, fruit hardness of whiteflesh fruit from Lanxi were 1.36, 1.096 and 1.22 times higher than those of Ruantiao white-flesh loquat from Huangyan, respectively. Flow cytometry analysis indicated that the two varieties were both diploid. Stomatal density of whiteflesh loquat from Lanxi was significantly higher than Ruantiao white-flesh loquat from Huangyan, while conversely, the stomatal area from Lanxi was significantly smaller than that from Huangyan. The cluster analysis using SSR marker showed that the genetic similarity coefficient (GS) of the loquat from Huangyan was 0.40, and 0.45 from Lanxi, indicating these two kinds of white-flesh loquats had a distant genetic relationship. The difference of the two varieties in maturation periods was mainly affected by variety characteristics and different geographical regions. 
In conclusion, the two experimental materials have a distant genetic relationship and belong to different germplasms. The creation of the novel germplasm has positive significance in enriching market supply and industrial development of white-flesh loquat.
收稿日期: 2016-01-21 出版日期: 2016-08-10
CLC:  S 667.3  
通讯作者: 陈方永(http://orcid.org/0000-0002-3134-9470),E-mail:cfy17266@126.com   
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引用本文:

陈方永, 王引, 倪海枝, 张启, 颜帮国. 浙江2个地产白沙枇杷种质资源亲缘关系鉴定[J]. 浙江大学学报(农业与生命科学版), 10.3785/j.issn.1008-9209.2016.01.211.

CHEN Fangyong, WANG Yin, NI Haizhi, ZHANG Qi, YAN Bangguo. Genetic relationship identification on germplasm resources of two whiteflesh loquats from two different producing areas of Zhejiang Province. Journal of Zhejiang University (Agriculture and Life Sciences), 10.3785/j.issn.1008-9209.2016.01.211.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2016.01.211        http://www.zjujournals.com/agr/CN/Y0/V/I/1

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